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首页> 外文期刊>Applied Microbiology >Phylogenetic and Functional Alterations in Bacterial Community Compositions in Broiler Ceca as a Result of Mannan Oligosaccharide Supplementation
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Phylogenetic and Functional Alterations in Bacterial Community Compositions in Broiler Ceca as a Result of Mannan Oligosaccharide Supplementation

机译：补充甘露寡糖对肉鸡盲肠细菌群落组成的系统发生和功能改变

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摘要

This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting. Two separate trials, each with a control and a supplemented group, were carried out in the same commercial location and run concurrently. Approximately 10,000 birds from the same commercial hatchery were mirror imaged into each of four commercial broiler sheds and fed either a control or supplemented diet. Cecal contents were obtained on days 7, 21, and 35 posthatch from 12 randomly caught broilers from each group. Bacterial pyrosequencing was performed on all samples, with approximately 250,000 sequences obtained per treatment per time point. The predominant phyla identified at all three time points in both trials were Firmicutes , Bacteroidetes , Proteobacteria , Actinobacteria , and Tenericutes , representing >99% of all sequences. MOS supplementation altered the bacterial community composition from 7 days supplementation through 35 days supplementation. Bacteroidetes appeared to be replacing Firmicutes as a result of supplementation, with the most noticeable effects after 35 days. The effects of supplementation were reproducible across both trials. PICRUSt was used to identify differences between the functional potentials of the bacterial communities as a result of MOS supplementation. Using level 3 KEGG ortholog function predictions, differences between control and supplemented groups were observed, with very strong segregation noted on day 35 posthatch in both trials. This indicated that alterations of bacterial communities as a result of MOS are likely to alter the functional capability of the cecum.
机译：这项研究的重点是在商业化生产环境中确定日粮添加甘露寡糖（MOS）对肉鸡盲肠细菌群落结构和功能的可再现作用。在相同的商业地点进行了两个单独的试验，每个试验都有一个对照组和一个补充组，并且同时进行。将来自同一商业孵化场的大约10,000只鸡镜像镜像到四个商业肉鸡舍的每个鸡舍中，并饲喂对照或补充饲料。在孵化后第7、21和35天从每组12只随机捕获的肉鸡中获取盲肠内容物。对所有样品进行细菌焦磷酸测序，每个处理时间每个点获得约250,000个序列。在两个试验中所有三个时间点都鉴定出的主要门系是Firmicutes，Bacteroidetes，Proteobacteria，Actinobacteria和Tenericutes，占所有序列的> 99％。 MOS的添加从7天的添加到35天的添加改变了细菌群落组成。由于补充，拟杆菌属似乎替代了硬菌类，在35天后效果最为明显。在这两个试验中补充的效果均可再现。 PICRUSt用于鉴定由于MOS补充而导致的细菌群落功能电位之间的差异。使用3级KEGG直系同源功能预测，观察到对照组和补充组之间的差异，两项试验均在孵化后第35天发现非常强烈的隔离。这表明由于MOS导致的细菌群落改变可能会改变盲肠的功能。

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