• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Deep-Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing Halanaerobium spp.
【24h】

Deep-Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing Halanaerobium spp.

机译：深地下压力刺激页岩定殖的哈拉纳霉菌属的代谢可塑性。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacterial Halanaerobium strains become the dominant persisting microbial community member in produced fluids across geographically distinct hydraulically fractured shales. Halanaerobium is believed to be inadvertently introduced into this environment during the drilling and fracturing process and must therefore tolerate large changes in pressure, temperature, and salinity. Here, we used a Halanaerobium strain isolated from a natural gas well in the Utica Point Pleasant formation to investigate metabolic and physiological responses to growth under high-pressure subsurface conditions. Laboratory incubations confirmed the ability of Halanaerobium congolense strain WG8 to grow under pressures representative of deep shale formations (21 to 48?MPa). Under these conditions, broad metabolic and physiological shifts were identified, including higher abundances of proteins associated with the production of extracellular polymeric substances. Confocal laser scanning microscopy indicated that extracellular polymeric substance (EPS) production was associated with greater cell aggregation when biomass was cultured at high pressure. Changes in Halanaerobium central carbon metabolism under the same conditions were inferred from nuclear magnetic resonance (NMR) and gas chromatography measurements, revealing large per-cell increases in production of ethanol, acetate, and propanol and cessation of hydrogen production. These metabolic shifts were associated with carbon flux through 1,2-propanediol in response to slower fluxes of carbon through stage 3 of glycolysis. Together, these results reveal the potential for bioclogging and corrosion (via organic acid fermentation products) associated with persistent Halanaerobium growth in deep, hydraulically fractured shale ecosystems, and offer new insights into cellular mechanisms that enable these strains to dominate deep-shale microbiomes.IMPORTANCE The hydraulic fracturing of deep-shale formations for hydrocarbon recovery accounts for approximately 60% of U.S. natural gas production. Microbial activity associated with this process is generally considered deleterious due to issues associated with sulfide production, microbially induced corrosion, and bioclogging in the subsurface. Here we demonstrate that a representative Halanaerobium species, frequently the dominant microbial taxon in hydraulically fractured shales, responds to pressures characteristic of the deep subsurface by shifting its metabolism to generate more corrosive organic acids and produce more polymeric substances that cause “clumping” of biomass. While the potential for increased corrosion of steel infrastructure and clogging of pores and fractures in the subsurface may significantly impact hydrocarbon recovery, these data also offer new insights for microbial control in these ecosystems.
机译：细菌Halanaerobium菌株成为横跨地理上不同的水力压裂页岩的采出液中主要的持久性微生物群落成员。人们认为在钻孔和压裂过程中无意中将卤化铌引入到该环境中，因此必须耐受压力，温度和盐度的较大变化。在这里，我们使用了从Utica Point Pleasant地层的天然气井中分离出的Halanaerobium菌株，以研究在高压地下条件下对生长的代谢和生理响应。实验室温育证实了Halanerobium congolense菌株WG8在代表深页岩地层（21至48？MPa）的压力下具有生长的能力。在这些条件下，鉴定出广泛的代谢和生理变化，包括与细胞外聚合物质产生相关的蛋白质的更高丰度。共聚焦激光扫描显微镜表明，当在高压下培养生物质时，细胞外聚合物（EPS）的产生与更大的细胞聚集有关。从相同的条件下，通过核磁共振（NMR）和气相色谱测量可以推断出卤化碳中枢碳代谢的变化，表明乙醇，乙酸盐和丙醇产量的每细胞大量增加，并且制氢停止。这些代谢变化与通过1,2-丙二醇的碳通量有关，以响应通过糖酵解阶段3的较慢的碳通量。总之，这些结果揭示了在水力压裂的深层页岩生态系统中，与卤素藻持续生长相关的生物阻塞和腐蚀（通过有机酸发酵产物）的潜力，并为使这些菌株在深层页岩微生物群落中占主导地位的细胞机制提供了新见解。用于开采碳氢化合物的深页岩地层的水力压裂约占美国天然气产量的60％。由于与硫化物的产生，微生物引起的腐蚀和地下生物堵塞相关的问题，与该过程相关的微生物活性通常被认为是有害的。在这里，我们证明了代表性的Hal藻属物种，通常是水力压裂页岩中的优势微生物分类群，通过改变其新陈代谢以产生更多腐蚀性有机酸并产生更多引起生物质“结块”的聚合物质，来响应深地下特征压力。尽管增加钢基础设施的腐蚀以及地下孔和裂缝的堵塞的可能性可能会显着影响碳氢化合物的回收，但这些数据也为这些生态系统中的微生物控制提供了新的见识。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号