...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Global Gene Expression Profile for Swarming Bacillus cereus Bacteria
【24h】

Global Gene Expression Profile for Swarming Bacillus cereus Bacteria

机译：蜡状芽孢杆菌的全球基因表达谱

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacillus cereus can use swarming to move over and colonize solid surfaces in different environments. This kind of motility is a collective behavior accompanied by the production of long and hyperflagellate swarm cells. In this study, the genome-wide transcriptional response of B. cereus ATCC 14579 during swarming was analyzed. Swarming was shown to trigger the differential expression (>2-fold change) of 118 genes. Downregulated genes included those required for basic cellular metabolism. In accordance with the hyperflagellate phenotype of the swarm cell, genes encoding flagellin were overexpressed. Some genes associated with K~(+) transport, phBC6A51 phage genes, and the binding component of the enterotoxin hemolysin BL (HBL) were also induced. Quantitative reverse transcription-PCR (qRT-PCR) experiments indicated an almost 2-fold upregulation of the entire hbl operon during swarming. Finally, BC1435 and BC1436, orthologs of liaI-liaH that are known to be involved in the resistance of Bacillus subtilis to daptomycin, were upregulated under swarming conditions. Accordingly, phenotypic assays showed reduced susceptibility of swarming B. cereus cells to daptomycin, and P_( spac )-induced hyper-expression of these genes in liquid medium highlighted the role of BC1435 and BC1436 in the response of B. cereus to daptomycin.
机译：蜡状芽孢杆菌可利用群体在不同环境中移动并定殖在固体表面上。这种运动是一种集体行为，伴随着长而鞭毛的群细胞的产生。在这项研究中，分析了蜂群中蜡状芽孢杆菌ATCC 14579的全基因组转录反应。研究表明，群体分化可以触发118个基因的差异表达（> 2倍变化）。下调的基因包括基本细胞代谢所需的基因。根据群细胞的超鞭毛表型，编码鞭毛蛋白的基因被过表达。还诱导了一些与K〜（+）转运相关的基因，phBC6A51噬菌体基因以及肠毒素溶血素BL（HBL）的结合成分。定量逆转录-PCR（qRT-PCR）实验表明，在蜂拥过程中整个hbl操纵子几乎上调了2倍。最后，BC1435和BC1436，liaI-liaH的直系同源物，已知与枯草芽孢杆菌对达托霉素的抗性有关，在成群条件下被上调。因此，表型测定显示成群的蜡状芽胞杆菌细胞对达托霉素的敏感性降低，并且P_（spac）诱导的这些基因在液体培养基中的过表达突出了BC1435和BC1436在蜡状芽孢杆菌对达托霉素的应答中的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号