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首页> 外文期刊>Applied Microbiology >Diversity of Salmonella Isolates from Central Florida Surface Waters
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Diversity of Salmonella Isolates from Central Florida Surface Waters

机译：来自佛罗里达州中部地表水的沙门氏菌的多样性

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Identification of Salmonella serotypes is important for understanding the environmental diversity of the genus Salmonella . This study evaluates the diversity of Salmonella isolates recovered from 165 of 202 Central Florida surface water samples and investigates whether the serotype of the environmental Salmonella isolates can be predicted by a previously published multiplex PCR assay (S. Kim, J. G. Frye, J. Hu, P. J. Fedorka-Cray, R. Gautom, and D. S. Boyle, J. Clin. Microbiol. 44:3608–3615, 2006, http://dx.doi.org/10.1128/JCM.00701-06). Multiplex PCR was performed on 562 Salmonella isolates (as many as 36 isolates per water sample) to predict serotypes. Kauffmann-White serogrouping was used to confirm multiplex PCR pattern groupings before isolates were serotyped, analyzed by pulsed-field gel electrophoresis, and assayed for antimicrobial susceptibility. In 41.2% of the Salmonella -positive water samples, all Salmonella isolates had identical multiplex PCR patterns; in the remaining 58.8%, two or more multiplex PCR patterns were identified. Within each sample, isolates with matching multiplex PCR patterns had matching serogroups. The multiplex patterns of 495 isolates (88.1%) did not match any previously reported pattern. The remaining 68 isolates matched reported patterns but did not match the serotypes for those patterns. The use of the multiplex PCR allowed the number of isolates requiring further analysis to be reduced to 223. Thirty-three Salmonella enterica serotypes were identified; the most frequent included serotypes Muenchen, Rubislaw, Anatum, Gaminara, and IV_50:z4,z23:?. A majority (141/223) of Salmonella isolates clustered into one genotypic group. Salmonella isolates in Central Florida surface waters are serotypically, genotypically, and phenotypically (in terms of antimicrobial susceptibility) diverse. While isolates could be grouped as different or potentially the same using multiplex PCR, the multiplex PCR pattern did not predict the Salmonella serotype.
机译：沙门氏菌血清型的鉴定对于理解沙门氏菌属的环境多样性很重要。这项研究评估了从202个佛罗里达州中部地表水样中的165个中回收的沙门氏菌的多样性，并调查了是否可以通过先前发表的多重PCR分析法预测环境沙门氏菌的血清型（S.Kim，JG Frye，J.Hu， PJ Fedorka-Cray，R. Gautom和DS Boyle，J. Clin。Microbiol。44：3608–3615，2006，http://dx.doi.org/10.1128/JCM.00701-06）。对562株沙门氏菌（每个水样多达36株）进行多重PCR以预测血清型。在对分离物进行血清分型，通过脉冲场凝胶电泳进行分析并测定其药敏性之前，使用Kauffmann-White血清分组法来确认多重PCR模式分组。在41.2％的沙门氏菌阳性水样中，所有沙门氏菌分离物均具有相同的多重PCR模式。在其余的58.8％中，鉴定出两个或多个多重PCR模式。在每个样品中，具有匹配的多重PCR模式的分离株具有匹配的血清群。 495个分离株（88.1％）的多重模式与以前报道的任何模式都不匹配。其余68株分离株与报道的模式匹配，但与这些模式的血清型不匹配。多重PCR的使用使需要进一步分析的分离株数量减少到223。鉴定了33种肠炎沙门氏菌血清型;其中3种被检出。最常见的血清型包括慕尼黑，鲁比斯拉夫，安纳塔姆，加米纳拉和IV_50：z4，z23：？。大部分（141/223）沙门氏菌分离株聚为一个基因型组。佛罗里达州中部地表水的沙门氏菌分离物在血清型，基因型和表型上（就抗菌药敏感性而言）是多种多样的。尽管可以使用多重PCR将分离株分组为不同或可能相同，但多重PCR模式无法预测沙门氏菌血清型。

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