...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Extraction, Purification, and Chemical and Immunological Properties of the Streptococcus mutans Group “a” Polysaccharide Cell Wall Antigen
【24h】

Extraction, Purification, and Chemical and Immunological Properties of the Streptococcus mutans Group “a” Polysaccharide Cell Wall Antigen

机译：变形链球菌“ a”多糖细胞壁抗原的提取，纯化以及化学和免疫学性质

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An antigen of Streptococcus mutans has been extracted from HS6 (group “a”) whole cells and repeatedly fractionated by Sephadex chromatography. The antigen is shown to be a polysaccharide and contains the S. mutans group “a” antigenic site and also a second antigenic site which is common to “a” strains and 2 of 3 group “d” strains. Immunological electrophoretic and chromatographic data indicate that the two sites exist in a single molecule. The polysaccharide has a molecular weight of 107,000 and is composed of glucose, galactose, glucosamine, and galactosamine. No significant quantities of lipid, phosphorus, glycerol, or ribitol are present. Immunological specificity of the group “a” polysaccharide site depends primarily on a d-glucose · d-glucose sequence, the “a-d” site on a terminal d-galactose. Water at 100 C and pepsin (pH 2.5) at room temperature are very effective in extracting the polysaccharide from lyophilized S. mutans cells. Trypsin and lysozyme are less effective. The antigen-antibody combining site appears to be located at the cell wall surface. A small quantity of enzyme-resistant protein (5%) is firmly linked to the antigen and is considered to be a remnant of a protein to which the polysaccharide is attached in the cell wall. The composition of the protein does not identify it as a part of the peptidoglycan. No reaction to the purified polysaccharide is obtained with antisera specific for teichoic acid glycerophosphate polymers from streptococci, staphylococci, or lactobacilli.
机译：已从HS6（“ a”组）全细胞中提取了变形链球菌的抗原，并通过Sephadex色谱法反复分离。抗原显示为多糖，并含有 S。 mutans 组的“ a”抗原位点以及“ a”株和3组“ d”株中的2个共有的第二个抗原位点。免疫电泳和色谱数据表明这两个位点存在于单个分子中。多糖的分子量为107,000，由葡萄糖，半乳糖，葡萄糖胺和半乳糖胺组成。没有大量的脂质，磷，甘油或核糖醇存在。 “ a”多糖位点的免疫学特异性主要取决于d-葡萄糖·d-葡萄糖序列，即末端d-半乳糖上的“ a-d”位点。 100°C的水和室温下的胃蛋白酶（pH 2.5）对于从冻干的 S中提取多糖非常有效。变形细胞。胰蛋白酶和溶菌酶效果较差。抗原-抗体结合位点似乎位于细胞壁表面。少量抗酶蛋白（5％）与抗原牢固连接，被认为是细胞壁中附着有多糖的蛋白的残留物。蛋白质的组成不能将其鉴定为肽聚糖的一部分。用来自链球菌，葡萄球菌或乳杆菌的对硫磷酸甘油磷酸酯聚合物具有特异性的抗血清，无法与纯化的多糖反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号