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首页> 外文期刊>Infection and immunity >Infection at the Subcellular Level II. Distribution and Fate of Intravenously Injected Brucellae Within Phagocytic Cells of Guinea Pigs
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Infection at the Subcellular Level II. Distribution and Fate of Intravenously Injected Brucellae Within Phagocytic Cells of Guinea Pigs

机译：在亚细胞水平II感染。豚鼠吞噬细胞内静脉注射布鲁氏菌的分布和命运

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Cells of Brucella melitensis strain 16 M were labeled with 32P. When injected into normal guinea pigs, labeled, viable bacteria were taken up and inactivated in liver and spleen during the 60 min after infection. Both uptake and inactivation increased if brucellae were coated with antibrucella antibody. Neither viability nor radioactivity were lost when labeled brucellae were incubated for 60 min in vitro with normal guinea pig blood, liver homogenates, or in defined medium. Incubation for 12 h with antibrucella rabbit immunoglobulin G similarly was innocuous. Livers were removed from infected animals at various times up to 60 min after injection and were separated into subcellular fractions. The numbers of total (determined by radioactivity measurements) and viable brucellae as well as the acid phosphatase activity in the various fractions were determined. Total bacteria and acid phosphatase activity were progressively transferred from the mitochondrial plus light mitochondrial (M + L) fraction to the nuclear (N) fraction. Viability of brucellae declined more rapidly in the N fraction than in other fractions. Examination of M + L fractions by isopycnic centrifugation showed a decrease in viability of both free brucellae and those in particles. The results indicated the formation of bacteria-containing heterolysosomes which progressively increased in size and in which brucellae were inactivated. The antibrucella activity of phagocytes of guinea pig liver in vivo appeared to be greater than that of peritoneal macrophages from immune rabbits or of bovine leukocytes studied in vitro.
机译：用 32 P标记布鲁氏菌菌株16 M的细胞。当将其注射到正常的豚鼠中后，在感染后60分钟内，被标记的活细菌就会被吸收并使其在肝和脾中失活。如果布鲁氏菌被抗小球藻抗体包被，则摄取和失活都会增加。当将标记的布鲁氏菌与正常的豚鼠血液，肝匀浆或在确定的培养基中体外孵育60分钟时，活力和放射性都不会丧失。用抗小球藻家兔免疫球蛋白G孵育12小时同样无害。在注射后直至60分钟的不同时间，从感染的动物中移出肝脏，并分离成亚细胞级分。确定了各个馏分中的总数（通过放射性测量确定）和存活的布鲁氏菌以及酸性磷酸酶活性。总细菌和酸性磷酸酶活性逐渐从线粒体和轻质线粒体（M + L）级分转移到核（N）级分。在N组分中，布鲁氏菌的生存力下降速度快于其他组分。通过等温离心法检测M + L馏分显示，游离布鲁氏菌和颗粒中的活力均降低。结果表明形成了含细菌的杂酶体，其尺寸逐渐增加并且布鲁氏菌被灭活。豚鼠体内吞噬细胞的抗小球藻活性似乎要比免疫兔腹腔巨噬细胞或体外研究的牛白细胞的抗小肠杆菌活性强。

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