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首页> 外文期刊>Infection and immunity >Blastogenesis and Lymphokine Synthesis by T and B Lymphocytes from Patients with Periodontal Disease
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Blastogenesis and Lymphokine Synthesis by T and B Lymphocytes from Patients with Periodontal Disease

机译：牙周病患者T淋巴细胞和B淋巴细胞的成胚和淋巴因子合成

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Thymus-derived (T) and bone marrow-derived (B) lymphocytes were isolated from human peripheral blood and cultured with various mitogens and antigens. Purified protein derivative of tuberculin stimulated both purified T and B cells from patients with positive skin reactivity to purified protein derivative but did not stimulate nonimmune lymphocytes. Similarly, both T and B lymphocytes from patients with periodontal disease were stimulated to proliferate when incubated with dental plaque, whereas cells from normal individuals without gingivitis were unresponsive. In contrast, one component of plaque, bacterial endotoxins (lipopolysaccharide), minimally stimulated B lymphocytes from both normal or gingivitis patients. T lymphocytes from patients with periodontal disease were also stimulated by plaque antigen to produce chemotactic lymphokine activity (CTX) for human monocytes. B cells purified by the EAC rosetting method nonspecifically produced CTX without concomitant blastogenesis; however, after dissociation of adherent EAC these immune B cells did not spontaneously produce CTX. Lymphokine synthesis by B cells was not dependent on concomitant blastogenesis. Dissociated B cells from periodontitis patients also produced CTX activity after stimulation with dental plaque antigen. Therefore, both T and B lymphocytes, after stimulation with nonendotoxin antigenic components of plaque, proliferated and produced lymphokines, which are presumed to contribute to the pathogenesis of periodontal disease.
机译：从人外周血中分离出胸腺来源的（T）和骨髓来源的（B）淋巴细胞，并用各种促细胞分裂剂和抗原进行培养。结核菌素的纯化蛋白衍生物刺激了对纯化蛋白衍生物具有阳性皮肤反应性的患者的纯化T和B细胞，但未刺激非免疫淋巴细胞。同样，与牙菌斑一起孵育时，来自牙周疾病患者的T和B淋巴细胞均被刺激增殖，而没有牙龈炎的正常个体的细胞则无反应。相比之下，斑块的一种成分，细菌内毒素（脂多糖），对正常或牙龈炎患者的B淋巴细胞的刺激作用最小。牙菌斑抗原还刺激来自牙周疾病患者的T淋巴细胞，从而产生人单核细胞趋化性淋巴因子活性（CTX）。通过EAC花环法纯化的B细胞非特异性地产生CTX，而没有伴随的胚发生。但是，在分离附着的EAC之后，这些免疫B细胞不会自发产生CTX。 B细胞的淋巴因子合成并不依赖于伴随的胚发生。来自牙周炎患者的分离的B细胞在用牙菌斑抗原刺激后也产生了CTX活性。因此，在用斑块的非内毒素抗原成分刺激后，T和B淋巴细胞均增殖并产生了淋巴因子，据推测这与牙周疾病的发病机理有关。

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