Affinity chromatography purification of Clostridium perfringens enterotoxin.

V N Scott; C L Duncan

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Affinity chromatography purification of Clostridium perfringens enterotoxin.

机译：产气荚膜梭菌肠毒素的亲和色谱纯化。

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Anti-enterotoxin immunoglobulins immobilized on CH-Sepharose or CNBr-Sepharose were used for affinity chromatography purification of Clostridium perfringens enterotoxin. Cell extracts containing enterotoxin or partially purified toxin preparations were applied to the column and nonspecifically-bound protein was eluted. NaOH was used to elute specifically bound toxin. The purity of enterotoxin purified by Sephadex G-100 chromatography followed by affinity chromatography appears similar to toxin highly purified by conventional means. The procedure can be used successfully for the rapid (less than 2 h) purification of small amounts of enterotoxin.

机译：固定在CH-Sepharose或CNBr-Sepharose上的抗肠毒素免疫球蛋白用于产气荚膜梭菌肠毒素的亲和层析纯化。将含有肠毒素或部分纯化的毒素制剂的细胞提取物上样至色谱柱，并洗脱非特异性结合的蛋白质。用NaOH洗脱特异性结合的毒素。通过Sephadex G-100色谱法和亲和色谱法纯化的肠毒素的纯度与通过常规手段高度纯化的毒素相似。该程序可成功用于少量肠毒素的快速纯化（少于2小时）。

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《Infection and immunity》 |1975年第3期|共8页
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V N Scott; C L Duncan;
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中图分类医学免疫学;
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专利

1. Preparative polyacrylamide gel electrophoresis purification of Clostridium perfringens enterotoxin. [J] . G L Enders, C L Duncan Infection and immunity . 1977,第2期

机译：产气荚膜梭菌肠毒素的制备型聚丙烯酰胺凝胶电泳纯化。
2. Eradication of chemotherapy-resistant CD44+ human ovarian cancer stem cells in mice by intraperitoneal administration of Clostridium perfringens enterotoxin. [J] . Casagrande F, Cocco E, Bellone S, Cancer: A Journal of the American Cancer Society . 2011,第24期

机译：通过腹膜内注射产气荚膜梭状芽胞杆菌肠毒素根除小鼠抗化疗的CD44 +人卵巢癌干细胞。
3. Claudin-4-targeting of diphtheria toxin fragment A using a C-terminal fragment of Clostridium perfringens enterotoxin. [J] . Kakutani H, Kondoh M, Saeki R, European journal of pharmaceutics and biopharmaceutics: official journal of Arbeitsgemeinschaft fuer Pharmazeutische Verfahrenstechnik e.V . 2010,第2期

机译：使用产气荚膜梭状芽胞杆菌肠毒素的C末端片段对白喉毒素片段A进行Claudin-4靶向。
4. Rapid, Specific, and Efficient Affinity Purification of Target Molecules by Combining Isotachophoresis and Affinity Chromatography(PPT) [C] . Viktor Shkolnikov, Juan G. Santiago AIChE Annual Meeting . 2014

机译：通过组合同位变和亲和层析（PPT）来快速，具体，高效地纯化靶分子（PPT）
5. Contribution of complex formation in the in vitro and in vivo action of Clostridium perfringens enterotoxin. [D] . Caserta, Justin Angelo. 2010

机译：产气荚膜梭菌肠毒素的体外和体内作用中复合物形成的贡献。
6. Affinity chromatography purification of Clostridium perfringens enterotoxin. [O] . V N Scott, C L Duncan 1975

机译：产气荚膜梭菌肠毒素的亲和色谱纯化。
7. Affinity Chromatography Purification of Clostridium perfringens Enterotoxin [O] . 1975

机译：亲和层析纯净梭菌净化肠肠毒素
8. Extraction, Purification and Characterization of a Peptidoglycan Hydrolase fromVegetative Cells of Clostridium perfringens Type A [R] . Labbe, R. G., Lu, H. P., Tang, S. 1990

机译：a型产气荚膜梭菌移植细胞肽聚糖水解酶的提取，纯化和鉴定

Affinity chromatography purification of Clostridium perfringens enterotoxin.

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