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首页> 外文期刊>Infection and immunity >Preparative polyacrylamide gel electrophoresis purification of Clostridium perfringens enterotoxin.
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Preparative polyacrylamide gel electrophoresis purification of Clostridium perfringens enterotoxin.

机译：产气荚膜梭菌肠毒素的制备型聚丙烯酰胺凝胶电泳纯化。

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摘要

Preparative polyacrylamide gel electrophoresis has been used to purify the enterotoxin of Clostridium perfringens from Sephadex G-100 extracts. Purified toxin of high specific activity was eluted in 1 to 3 h, depending upon the length of the acrylamide gel used. Recovery of biological activity with this technique ranged from 80 to 90%. The purity and physical characteristics of the toxin were similar to those previously reported for the protein purified by other methods. Use of preparative electrophoresis will enable the production of larger amounts of high-specific-activity toxin in a shorter time than other currently available procedures. This method was also used to isolate a form of enterotoxin that has a mobility, relative to bromophenol blue tracking dye, of 0.87 to 0.90 in 7% acrylamide gels.
机译：制备型聚丙烯酰胺凝胶电泳已用于从Sephadex G-100提取物中纯化产气荚膜梭菌的肠毒素。根据所用丙烯酰胺凝胶的长度，可以在1-3小时内洗脱出具有高比活性的纯化毒素。使用该技术的生物活性恢复率为80％至90％。该毒素的纯度和物理特性与先前报道的通过其他方法纯化的蛋白质相似。制备电泳的使用将使比其他目前可用的方法在更短的时间内产生大量的高比活性毒素。此方法还用于分离一种形式的肠毒素，相对于溴酚蓝跟踪染料，该毒素在7％丙烯酰胺凝胶中的迁移率为0.87至0.90。

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