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首页> 外文期刊>Infection and immunity >Polymyxins as inhibitors of polyclonal B-cell activators in murine lymphocyte cultures.
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Polymyxins as inhibitors of polyclonal B-cell activators in murine lymphocyte cultures.

机译：多粘菌素作为鼠淋巴细胞培养物中多克隆B细胞激活剂的抑制剂。

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摘要

The polymyxin antibiotics polymyxin B sulfate and colistin methane sulfonate were examined for their ability to inhibit responses to the polyclonal B-cell activators (PBA) bacterial lipopolysaccharide (LPS), dextran sulfate (DS), pneumococcal polysaccharide (SIII), and purified protein derivative of tuberculin (PPD) in spleen cell cultures. Polymyxin concentrations of 1 and 10 microng/ml significantly inhibited both the deoxyribonucleic acid synthetic and polyclonal antibody responses stimulated by LPS, DS, and SIII. At these concentrations of polymyxins, responses to PPD and to the T-cell mitogens concanavalin A and phytohemagglutinin were not affected. Inhibition was not caused by a generalized lymphocyte toxicity. After dialysis of LPS-polymyxin and DS-polymyxin mixtures, the PBA preparations showed decreased mitogenic activity. Thus, the polymyxins probably interacted directly with the LPS and DS molecules. The mitogenic response to DS was more significantly inhibited than the response to a nonsulfated dextran. The cationic property of the polymyxins probably allows attachment to negatively charged groups in the mitogenically relevant parts of some but not all PBA molecules,, this attachment resulting in the loss of PBA activity.
机译：检查了多粘菌素抗生素多粘菌素B硫酸盐和粘菌素甲烷磺酸盐对多克隆B细胞激活剂（PBA）细菌脂多糖（LPS），硫酸葡聚糖（DS），肺炎球菌多糖（SIII）和纯化的蛋白衍生物的反应抑制能力脾细胞培养中的结核菌素（PPD）含量。 1和10 microng / ml的多粘菌素浓度可显着抑制LPS，DS和SIII刺激的脱氧核糖核酸合成和多克隆抗体反应。在这些多粘菌素浓度下，对PPD以及对T细胞丝裂原伴伴刀豆球蛋白A和植物血凝素的反应均不受影响。抑制不是由全身淋巴细胞毒性引起的。在透析LPS-多粘菌素和DS-多粘菌素混合物后，PBA制剂显示出有丝分裂活性降低。因此，多粘菌素可能直接与LPS和DS分子相互作用。对DS的促有丝分裂反应比对未硫酸化的右旋糖酐的反应有更显着的抑制作用。多粘菌素的阳离子性质可能允许其连接到某些而非全部PBA分子的有丝分裂相关部分中的带负电基团，这种连接导致PBA活性降低。

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