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首页> 外文期刊>Infection and immunity >Demonstration of Serologically Different Capsular Types Among Strains of Staphylococcus aureus by the Serum-Soft Agar Technique
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Demonstration of Serologically Different Capsular Types Among Strains of Staphylococcus aureus by the Serum-Soft Agar Technique

机译：血清软琼脂技术在金黄色葡萄球菌菌株中血清学上不同的荚膜类型的证明

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Colonies of Staphylococcus aureus exhibiting diffuse-type growth in regular serum-soft agar containing 7.5% sodium chloride were isolated. After isolation, further identification of the encapsulated strains of S. aureus was performed. With this procedure, 19 encapsulated strains were obtained from 103 clinical specimens (18.4%). With these strains, three serologically distinct diffuse types of organisms were observed by the conversion of diffuse to compact type colonial morphology in serum-soft agar containing specific antidiffuse sera. Capsule-inhibiting activity of antisera was adsorbable with homologous encapsulated organisms and not adsorbed with either heterologous encapsulated organisms nor the derived compact variant, suggesting a specific activity for the antispecific capsular antibody. Fourteen strains were similar to the Smith diffuse-type strain, four strains were the same as NS58D, and one was identical to NS41D. These were provisionally designated as capsule types A, B, and C, respectively.
机译：分离出金黄色葡萄球菌的菌落，在含有7.5％氯化钠的普通血清-软琼脂中表现出弥散型生长。分离后，进一步鉴定 S的封装菌株。进行了金黄色操作。通过此程序，从103个临床标本中获得了19株包囊菌株（占18.4％）。在这些菌株中，通过在含有特定抗扩散血清的血清软琼脂中将扩散转变为紧凑型菌落形态，观察到了三种血清学上不同的扩散型生物。抗血清的胶囊抑制活性可被同源的被包封的生物体吸附，而未被异源的被包封的生物体或衍生的致密变体吸附，这表明抗特异性荚膜抗体具有特异性活性。十四个菌株与史密斯弥散型菌株相似，四个菌株与NS58D相同，一个与NS41D相同。这些被分别临时指定为胶囊类型A，B和C。

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