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Characteristics of Complement-Dependent Release of Phospholipid from Escherichia coli

机译：从大肠杆菌中补体依赖性释放磷脂的特征

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The release of 32P-labeled bacterial phospholipid from a smooth Escherichia coli by serum components depends on complement activated by antibody. Phospholipid release in excess antibody tends to be proportional to the concentration of complement as does the release of other cellular constituents. Phospholipids are not simply stripped off during cell lysis. Whereas 94% of the total phospholipid freed from E. coli by mechanical lysis sediments at centrifugal forces sufficient to sediment molecules of 106 molecular weight, similar centrifugation sediments only 50% of the phospholipid released by antibody-complement. In fact, after mechanical lysis more than 50% of the phospholipid sediments at velocities sufficient to bring down cell envelopes. Although the bulk of the bacterial phospholipid is located in the cell envelopes, isolated 32P-labeled cell envelopes and phenol-extracted lipopolysaccharide fails to release phospholipids in the presence of antibody-complement. Moreover, ethylenediaminetetraacetic acid, which like antibody-complement causes loss of cellular selective permeability and prepares E. coli cell walls for the action of lysozyme, releases only small amounts of phospholipid from E. coli and these are sedimentable. The most likely mechanism of phospholipid release caused by antibody-complement appears to be the activation directly or indirectly of an enzyme which is present only in the intact cells.
机译：血清成分从光滑的大肠杆菌中释放 32 P标记的细菌磷脂取决于抗体激活的补体。过量抗体中的磷脂释放往往与补体浓度成正比，其他细胞成分的释放也是如此。磷脂在细胞裂解过程中不会简单地剥离。而总磷脂中有94％从E中释放出来。通过机械裂解作用在大肠杆菌中沉淀物，离心力足以沉淀分子量为10 6 的分子，类似的离心作用只能沉淀抗体补体释放的磷脂的50％。实际上，在机械裂解后，超过50％的磷脂沉积物以足以降低细胞膜的速度沉积。尽管大部分细菌磷脂位于细胞包膜中，但在抗体补体存在下，分离的 32 P标记的细胞包膜和苯酚提取的脂多糖无法释放磷脂。此外，像抗体补体一样的乙二胺四乙酸会引起细胞选择性通透性的损失并制备E。溶菌酶作用的大肠杆菌细胞壁仅从 E中释放少量磷脂。大肠杆菌，它们是可沉积的。由抗体补体引起的磷脂释放的最可能机制似乎是直接或间接激活仅存在于完整细胞中的酶。

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