Rapid Batch Method for the Preparation of Fluorescein Isothiocyanate-Conjugated Antibody

John V. Calcagno; Michael J. Sweeney; Helen C. Oels

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Rapid Batch Method for the Preparation of Fluorescein Isothiocyanate-Conjugated Antibody

机译：快速分批法制备荧光素异硫氰酸酯偶联抗体

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A rapid, reproducible, and relatively simple method for the preparation of large quantities of fluorescein isothiocyanate-conjugated antibody is presented. Tagged globulins with fluorochrome-protein ratios of 1 and 2 were eluted from diethylaminoethyl-cellulose in batch form by using sodium phosphate-buffered 0.1 M and 0.2 M saline, respectively. The intensity and specificity of the fluorescence due to this antibody was quite high, as determined by the ability of untagged antibody to block the immunological reactivity of the fluorescent antibody and by the lack of fluorescence of antigenically unrelated material when treated under identical conditions.

机译：提出了一种快速，可重现和相对简单的制备大量荧光素异硫氰酸酯偶联抗体的方法。分别使用磷酸钠缓冲的0.1 M和0.2 M盐水从二乙氨基乙基纤维素中分批洗脱出具有荧光色素-蛋白质比率为1和2的标记球蛋白。由该抗体引起的荧光的强度和特异性很高，这是由未标记的抗体阻断荧光抗体的免疫反应性的能力以及在相同条件下处理时抗原无关材料的荧光不足所确定的。

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《Infection and immunity》 |1973年第3期|共4页
作者
John V. Calcagno; Michael J. Sweeney; Helen C. Oels;
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中图分类医学免疫学;
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1. Evaluation of a solid-phase immunoassay with fluorescein isothiocyanate-conjugated heterogeneous or monoclonal antibodies for identification of virus isolates, with influenza virus as a model. [J] . D J Phillips, G G Galland, C B Reimer, Journal of Clinical Microbiology . 1982,第5期

机译：以流感病毒为模型，评估与异硫氰酸荧光素偶联的异源或单克隆抗体的固相免疫测定，以鉴定病毒分离物。
2. Rapid and simple method for double staining of bacteria with 4',6-diamidino-2-phenylindole and fluorescein isothiocyanate-labeled antibodies. [J] . K A Hoff Applied and Environmental Microbiology . 1988,第12期

机译：用4'，6-二氨基-2-苯基吲哚和荧光素异硫氰酸酯标记的抗体进行快速简便的方法。
3. Rapid Fluorescein and Protein Assay Method for Fluorescent-Antibody Conjugates [J] . Arthur F. Wells, Curtis E. Miller, Marvin K. Nadel Applied Microbiology . 1966,第2期

机译：荧光抗体结合物的快速荧光素和蛋白质测定方法
4. RAPID HPLC-MS/MS EVALUATION OF THE BATCH-TO-BATCH CONSISTENCY OF THE PAYLOADS SUBSTITUTION OF A LYSINE ANTIBODY-DRUG-CONJUGATES (ADCS) [C] . Fabiana Tavani, Antonio Triolo International Mass Spectrometry Conference . 2018

机译：快速HPLC-MS / MS评估批量载荷替代赖氨酸抗体 - 药物 - 缀合物（ADC）的批量替换
5. Rapid sample preparation methods for use with high-speed gas chromatography. [D] . Current, Robert Wesley. 1999

机译：快速样品制备方法，可用于高速气相色谱。
6. Rapid Batch Method for the Preparation of Fluorescein Isothiocyanate-Conjugated Antibody [O] . John V. Calcagno, Michael J. Sweeney, Helen C. Oels 1973

机译：快速分批法制备荧光素异硫氰酸酯偶联抗体
7. Rapid Batch Method for the Preparation of Fluorescein Isothiocyanate-Conjugated Antibody [O] . John V. Calcagno, Michael J. Sweeney, Helen C. Oels 1973

机译：制备荧光素异硫氰酸酯 - 缀合的抗体的快速分批方法
8. Preparation of Mycoplasma Seed Antigen, Antiserum, and Fluorescein Labeled Antibody Reagents and Conduct of a Mycoplasma Detection and Identification Service [R] . Carski, T. R., Robillard, N. F., Delgiudice, R. A. 1969

机译：支原体种子抗原，抗血清和荧光素标记抗体试剂的制备及支原体检测和鉴定服务的实施

Rapid Batch Method for the Preparation of Fluorescein Isothiocyanate-Conjugated Antibody

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