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首页> 外文期刊>Infection and immunity >Factors Affecting Virulence of Shigella flexneri: Avirulent Strain with Altered Metabolism of Succinate, Fumarate, and Malate
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Factors Affecting Virulence of Shigella flexneri: Avirulent Strain with Altered Metabolism of Succinate, Fumarate, and Malate

机译：影响弗氏志贺氏菌毒力的因素：琥珀酸，富马酸和苹果酸代谢改变的无毒菌株。

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摘要

It has been found that a mutation of Shigella flexneri 2a to avirulence can result in altered metabolism of tricarboxylic acid cycle acids, but the effect is strongly dependent on growth conditions. Mutant cells harvested from stationary-phase cultures grown with vigorous shaking in nutrient broth oxidize succinate, fumarate, and malate much more slowly than do wild-type cells. Cell-free extracts from such cells show similar differences in oxidative ability. These differences were less pronounced when cells were harvested from the exponential phase of growth and were nonexistent when the cells were grown without shaking. When tricarboxylic acid cycle acids were used as carbon sources with shaking, mutant cells from exponential phase cultures oxidized tricarboxylic cycle acids almost to the same extent as the wild type. Stationary-phase cells grown on succinate lost succinoxidase activity, but wild-type cells retained more than twice the activity of mutant cells. The effect of growth conditions on oxidative differences between the two strains was similar to the differences in succinate uptake observed under similar conditions.
机译：已经发现，弗氏志贺氏菌 2a突变为无毒力可导致三羧酸循环酸代谢的改变，但其作用强烈取决于生长条件。从营养培养液中剧烈振摇生长的固定相培养物中收获的突变细胞，其氧化琥珀酸，富马酸和苹果酸的速度比野生型细胞慢得多。来自此类细胞的无细胞提取物在氧化能力上显示出相似的差异。当从指数生长期收获细胞时，这些差异不太明显，而当细胞不摇动生长时则不存在。当将三羧酸循环酸摇动用作碳源时，来自指数相培养物的突变细胞氧化三羧酸循环酸的程度几乎与野生型相同。在琥珀酸盐上生长的静止期细胞丧失了琥珀氧化酶活性，但野生型细胞保留了突变细胞两倍以上的活性。生长条件对两个菌株之间氧化差异的影响类似于在相似条件下观察到的琥珀酸吸收差异。

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