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首页> 外文期刊>Infection and immunity >Action of Escherichia coli Enterotoxin: Adenylate Cyclase Behavior of Intestinal Epithelial Cells in Culture
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Action of Escherichia coli Enterotoxin: Adenylate Cyclase Behavior of Intestinal Epithelial Cells in Culture

机译：大肠杆菌肠毒素的作用：培养中肠上皮细胞的腺苷酸环化酶行为

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Heat-labile enterotoxin preparations obtained from two enteropathogenic strains of Escherichia coli of porcine and human origin were shown to stimulate adenylate cyclase activity of human embryonic intestinal epithelial cells in culture. Comparable results were also obtained when cholera toxin was used. The degree of enzyme stimulation was proportional to the concentration of enterotoxin. Similar preparations from two strains of non-enterotoxigenic E. coli had no effect on adenylate cyclase activity. Cells exposed to enterotoxin could be washed after 1 min of contact time without altering the subsequent course of maximum adenylate cyclase activity, which was maintained for at least 18 h at 37 C. During long periods (18 h) of tissue culture incubation, the determination of adenylate cyclase activity was 200- to 300-fold more sensitive than quantitating fluid accumulation in the adult rabbit ileal loop model. Decreasing the incubation time appreciably reduced the sensitivity of the epithelial cells to enterotoxin. E. coli enterotoxin is an effective activator of nonintestinal adenylate cyclase systems. Treatment of KB and HEp-2 cell lines with enterotoxin also resulted in significant enzyme stimulation. The intestinal epithelial cell tissue culture model provides a sensitive homogenous biological system for studying the response of intestinal adenylate cyclase to enterotoxin while eliminating the numerous cellular and tissue components present in the ligated ileal loop model.
机译：从猪和人源的两个大肠杆菌病原菌株中获得的不耐热肠毒素制剂可刺激培养的人胚肠上皮细胞的腺苷酸环化酶活性。当使用霍乱毒素时，也获得了可比的结果。酶刺激的程度与肠毒素的浓度成正比。从两种非肠毒素的 E菌株制备相似的制剂。大肠杆菌对腺苷酸环化酶活性没有影响。接触肠毒素的细胞可在接触时间1分钟后洗涤，而不会改变随后最大腺苷酸环化酶活性的过程，该过程在37°C保持至少18h。在组织培养的长时间（18h）中，测定在成年兔回肠loop模型中，腺苷酸环化酶活性的敏感性比定量液体积聚的敏感性高200至300倍。减少孵育时间会明显降低上皮细胞对肠毒素的敏感性。 E。肠毒素是非肠道腺苷酸环化酶系统的有效激活剂。用肠毒素处理KB和HEp-2细胞系也导致明显的酶刺激。肠上皮细胞组织培养模型为研究肠腺苷酸环化酶对肠毒素的反应提供了一个敏感的均质生物学系统，同时消除了在结扎回肠环模型中存在的众多细胞和组织成分。

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