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首页> 外文期刊>Infection and immunity >Cytotoxic effect of Mycoplasma fermentans on mouse thymocytes.
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Cytotoxic effect of Mycoplasma fermentans on mouse thymocytes.

机译：发酵支原体对小鼠胸腺细胞的细胞毒性作用。

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The in vitro response of mouse thymocytes to various mycoplasmas was evaluated. Cultures of thymus cells from BALB mice were prepared in Earle minimal essential medium with 10 per cent fetal calf serum. After an initial drop in viability, cell populations stabilized at approximately 10-5 viable cells/ml for 3 to 5 days. Concentration of 10-6 to 10-8 colony-forming units of toxic isolates of Mycoplasma fermentans per ml killed over 50 per cent of these cells in a dose-dependent fashion. Four other mycoplasmas (M. pneumoniae, M. hominis, M. arthritidis and a nontoxic strain of M. fermentans) did not induce cytotoxicity of mouse thymocytes. Toxic isolates of M. fermentans multiplied in the presence of thymus cells as they were being inactivated. However, nonviable membrane preparations of these mycoplasma were also toxic, indicating that growth of the organisms is not a prerequisite for the toxic effect. The relevance of these findings for the isolation and identification of the membrane-associated toxic factor is discussed.
机译：评价了小鼠胸腺细胞对各种支原体的体外反应。在含有10％胎牛血清的Earle基本必需培养基中制备BALB小鼠的胸腺细胞培养物。活力最初下降后，细胞群体稳定在大约10-5个活细胞/毫升，持续3至5天。每毫升浓度为10-6至10-8菌落发酵支原体的毒性分离菌落形成单位的浓度以剂量依赖性方式杀死了这些细胞的50％以上。其他四个支原体（肺炎支原体，人型支原体，关节炎支原体和发酵型支原体的无毒株）均未诱导小鼠胸腺细胞的细胞毒性。在胸腺细胞失活的情况下，发酵支原体的有毒分离物成倍增加。然而，这些支原体的不可行的膜制剂也是有毒的，表明生物体的生长不是毒性作用的先决条件。讨论了这些发现与膜相关毒性因子的分离和鉴定的相关性。

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