...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Indian Journal of Science and Technology >Goldfish Embryo Development after Somatic Cell Nuclear Transfer in Nonenucleated Oocytes: first Mitosis Profile, Ploidy Status, and Genetic Conformity
【24h】

Goldfish Embryo Development after Somatic Cell Nuclear Transfer in Nonenucleated Oocytes: first Mitosis Profile, Ploidy Status, and Genetic Conformity

机译：在无核卵母细胞中进行体细胞核转移后的金鱼胚胎发育：第一有丝分裂概况，倍性状态和遗传整合。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nuclear transfer has the potential to become onestrategy for fish genetic resources management, byallowing fish reconstruction from cryopreserved somaticcells. Survival rates after nuclear transfer are still lowhowever [5], both because of biological and technicalconstraints. Among them, the difficulty to enucleate therecipient oocyte in fish, together with the need toalleviate some of the alterations associated with theenucleation methods, led several authors to inject thedonor nucleus into non-enucleated oocytes [1,2,4].Strikingly, diploid clones were produced whichexhibited genetic characteristics of the donor fish. Thegroup of Wakamatsu demonstrated moreover thatdiploidized activated eggs were a good template for thedevelopment of somatic cell nuclear transplants [6],although the mechanism underlying a putativespontaneous erasure of the maternal genome remains tobe explored. In a previous work, we developed a nucleartransfer method in goldfish where the recipient oocyteswere at the metaphase II stage (non-activated) [3]. Asalso proposed by [5] and in several works in mammals, ametaphase II recipient should improve thereprogramming of the injected nucleus, by allowingbetter cell cycle synchronization between the donor andthe recipient.
机译：通过允许从低温保存的体细胞进行鱼类重建，核转移有可能成为鱼类遗传资源管理的战略。然而，由于生物学和技术的限制，核移植后的存活率仍然很低[5]。其中，鱼类去核卵母细胞去核的困难，以及减轻与去核方法有关的某些变化的需要，导致几位作者将供体核注入非去核卵母细胞中[1,2,4]。生产出具有供体鱼遗传特性的鱼。 Wakamatsu小组还证明了二倍体活化的卵是体细胞核移植发展的一个很好的模板[6]，尽管仍需要探索潜在的自发清除母体基因组的机制。在以前的工作中，我们在金鱼中开发了一种核转移方法，其中受体卵母细胞处于中期II期（未激活）[3]。 [5]也提出并在哺乳动物的一些工作中，通过允许供体和受体之间更好的细胞周期同步，中期II受体应改善注射核的程序设计。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号