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首页> 外文期刊>Infection and immunity >Immunofluorescent Study of the Replication of Infectious Pancreatic Necrosis Virus in Trout and Atlantic Salmon Cell Cultures
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Immunofluorescent Study of the Replication of Infectious Pancreatic Necrosis Virus in Trout and Atlantic Salmon Cell Cultures

机译：在鳟鱼和大西洋鲑鱼细胞培养物中复制感染性胰腺坏死病毒的免疫荧光研究。

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Cell cultures of trout gonad tissue (RTG-2) and Atlantic salmon heart, kidney, liver, and spleen tissue were inoculated with 50 50% tissue culture infective doses (TCID50) of infectious pancreatic necrosis (IPN) virus per cell, and the titer of cell-associated and released virus was determined from 2 to 16 h postinoculation (PI). Cover slips were collected over the same period and stained for IPN viral antigen by the direct immunofluorescent (FA) technique. Viral replication was detected after a latent period of approximately 2 to 4 h and reached a peak titer of 108.2 to 108.4 TCID50 per ml at 8 to 10 h PI. The release of virus was more rapid in Atlantic salmon cells than in RTG-2 cells. Viral antigen was first detected by FA from 3 to 4 h PI. Approximately 75 to 80% of the cells contained antigen in the cytoplasm 9 to 11 h PI. The direct FA technique was found to be a sensitive method for detecting IPN virus in infected cells. Three strains of IPN virus were tested for serological cross reactions by FA and virus neutralization tests.
机译：鳟鱼性腺组织（RTG-2）和大西洋鲑心，肾，肝和脾脏组织的细胞培养物中接种了50 50％感染性胰腺坏死（IPN）组织培养感染剂量（TCID 50 ）病毒/细胞），并在接种后2到16小时内确定与细胞相关和释放的病毒的滴度。在同一时期收集盖玻片，并通过直接免疫荧光（FA）技术对IPN病毒抗原染色。在大约2至4小时的潜伏期后检测到病毒复制，并达到每毫升10 8.2 至10 8.4 TCID 50 的峰值滴度在PI的8到10小时。大西洋鲑鱼细胞中的病毒释放比RTG-2细胞中的病毒释放更快。病毒抗原在PI 3至4 h时首先被FA检测。 9至11小时PI中，约75至80％的细胞在细胞质中包含抗原。发现直接FA技术是检测感染细胞中IPN病毒的灵敏方法。通过FA和病毒中和测试，测试了三株IPN病毒的血清学交叉反应。

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