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首页> 外文期刊>Infection and immunity >Penetration of Cultured Mouse Fibroblasts (L Cells) by Rickettsia prowazeki
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Penetration of Cultured Mouse Fibroblasts (L Cells) by Rickettsia prowazeki

机译：立克次氏菌对培养的小鼠成纤维细胞（L细胞）的渗透

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The association of Rickettsia prowazeki with L cells was examined by using a novel radioactive assay in which [α-32P]ATP-labeled rickettsiae were incubated with L-cell monolayers. Rickettsial association with the monolayer involved adherence and internalization steps that could be experimentally distinguished. Since R. prowazeki but not L cells possess an ATP-ADP obligate exchange transport system, addition of excess unlabeled ATP resulted in exchange of the labeled ATP from external, adherent rickettsiae but not from internalized rickettsiae. Rickettsial association was temperature dependent and was a linear function of both time and concentration. More than 90% of the biologically active rickettsiae associated with L cells was internalized. Rickettsial internalization required active participation of both rickettsiae and L cells; inactivation of either greatly reduced internalization. Rickettsial adherence to poisoned L cells was a saturable function of time and concentration. Adherence showed less temperature dependence than did internalization, but like rickettsial internalization, the extent of adherence was extremely low at 0°C. The rate and extent of adherence by inactivated and native rickettsiae to inactivated L cells were similar. Although inactive rickettsiae adhered to active and inactive L cells to a similar extent, inactive rickettsiae were internalized poorly by active L cells. These data form the basis for the hypothesis that R. prowazeki are internalized by the host cell through a process of “induced phagocytosis” and that inactivated rickettsiae adhere to the host cell differently from native rickettsiae, failing to trigger the endocytosis mechanism.
机译：用新的放射性分析法检测了 Rickettsia prowazeki 与L细胞的缔合，其中将[α- 32 P] ATP标记的立克次体与L细胞单层孵育。立克次体与单层的结合涉及粘附和内在化步骤，这可以通过实验加以区分。自 R。 Prowazeki （而非L细胞）具有ATP-ADP专性交换转运系统，添加过量的未标记ATP会导致来自外部粘附的立克次体的标记ATP交换，而不是来自内部化的立克次体的交换。立克次体关联是温度依赖性的，并且是时间和浓度的线性函数。超过90％的与L细胞相关的具有生物活性的立克次体被内在化。立克次体内在化需要立克次体和L细胞的积极参与。灭活要么大大减少了内部化。立克次体对中毒的L细胞的粘附是时间和浓度的饱和函数。附着力显示的温度依赖性低于内部化，但像立克次体内部化一样，在0°C时，附着力极低。灭活的和天然的立克次体对灭活的L细胞的粘附速率和程度相似。尽管非活性立克次体以相似的程度粘附于活性和非活性L细胞，但是非活性立克次体被活性L细胞内化的较弱。这些数据构成了 R假设的基础。 prowazeki 被宿主细胞通过“诱导吞噬”过程内在化，灭活的立克次体与天然立克次体的粘附方式不同，无法触发内吞作用。

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