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首页> 外文期刊>Infection and immunity >Selective Binding of Blood Group-Reactive Salivary Mucins by Streptococcus mutans and Other Oral Organisms
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Selective Binding of Blood Group-Reactive Salivary Mucins by Streptococcus mutans and Other Oral Organisms

机译：变形链球菌和其他口腔生物对血型反应性唾液黏蛋白的选择性结合

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Strains of Streptococcus mutans of four genetic groups and five serotypes, and strains of S. sanguis, S. mitis, S. salivarius, Actinomyces naeslundii, and A. viscosus, were found to bind blood group-reactive (BGR) mucin isolated from whole human saliva. The bacteria studied bound mucins with blood type A or B reactivity to a similar extent, suggesting that the carbohydrate moieties responsible for the A and B antigenic determinants were not involved. The organisms studied appeared to bind different fractions of BGR mucin molecules because preparations absorbed with cells of a given oral species no longer contained BGR molecules which bound to homologous organisms but still possessed BGR components which bound to varying degrees to other bacteria. Differences were even noted among S. mutans strains belonging to different genetic groups and serological types. Immunoglobulins could not be detected in the mucin preparations, and addition of anti-human immunoglobulin A (IgA), IgG, or IgM serum to reaction mixtures did not affect binding. Mucin pretreated with periodate or iodoacetate no longer bound to S. mutans H12 cells, suggesting that carbohydrate moieties and sulfhydryl groups played an essential role. Active cell metabolism was not required for BGR mucin binding; however, pretreatment of H12 cells with periodate or heat (100°C for 15 min) reduced binding. Mucin labeled with [14C]phenyl isothiocyanate appeared to bind to S. mutans H12 cells comparably to untreated mucin; the binding also appeared to be specific because less than 15% of the labeled material became bound when incubated with an excess of streptococci. Binding of [14C]phenyl isothiocyanate-labeled mucin was not affected by neutral sugars tested or by preparations of c antigen, glycerol teichoic acid, dextran, or crude glucosyltransferase. However, binding was inhibited by several amines. BGR salivary mucins are present in the acquired pellicle covering teeth; the ability of bacteria to selectively bind such components suggest that they may serve as receptor molecules involved in the attachment of bacteria to teeth.
机译：四个基因组和五个血清型的变形链球菌菌株及其菌株。 sanguis，S。mitis，S。salivarius，Naslundii放线菌和 A。粘液被发现与从整个人唾液中分离出的血型反应性粘蛋白结合。该细菌研究了结合的粘蛋白与血液A型或B型反应性的相似程度，这表明负责A和B抗原决定簇的碳水化合物部分不参与。研究的生物似乎结合了不同比例的BGR粘蛋白分子，因为在给定口腔物种的细胞中吸收的制剂不再包含与同源生物结合的BGR分子，但仍然具有与其他细菌不同程度结合的BGR成分。甚至在 S之间注意到差异。属于不同基因组和血清学类型的变异株。在粘蛋白制剂中未检测到免疫球蛋白，向反应混合物中添加抗人免疫球蛋白A（IgA），IgG或IgM血清不会影响结合。用高碘酸盐或碘乙酸盐预处理的粘蛋白不再与 S结合。变形的H12细胞，提示碳水化合物部分和巯基起着至关重要的作用。 BGR粘蛋白结合不需要活跃的细胞代谢。但是，用高碘酸盐或热（100°C加热15分钟）预处理H12细胞会降低结合。用[ 14 C]苯基异硫氰酸酯标记的粘蛋白似乎与 S结合。 H12细胞与未经处理的粘蛋白相当;这种结合似乎也是特异性的，因为当与过量的链球菌一起孵育时，不到15％的标记物质被结合。 [ 14 C]苯基异硫氰酸酯标记的粘蛋白的结合不受所测试的中性糖或c抗原，甘油海藻酸，葡聚糖或粗制葡萄糖基转移酶制剂的影响。但是，结合被几种胺抑制。 BGR唾液粘蛋白​​存在于获得的防护膜覆盖的牙齿中;细菌选择性结合这些成分的能力表明，它们可以作为参与细菌附着在牙齿上的受体分子。

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