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Occurrence of Protein A in Staphylococcal Strains: Quantitative Aspects and Correlation to Antigenic and Bacteriophage Types

机译:葡萄球菌菌株中蛋白A的出现:定量方面和与抗原和噬菌体类型的相关性。

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Protein A of Staphylococcus aureus can be detected on cell walls of intact bacteria by use of radioactively labeled myeloma globulin. Of 156 strains of S. aureus, 141 (90%) contained protein A. None of 47 S. epidermidis strains was positive for protein A. The production of protein A was influenced by incubation temperature but not by differences in incubation time or inoculum size. A medium containing a high concentration of NaCl suppressed the production of protein A by 90%. Formalin treatment of protein A-containing strains caused a decrease in the amount detected, but no further decrease was detected after storage at 4 C. No correlation was found between absence or presence of protein A and phage type or phage group. Sixteen S. aureus strains were studied extensively. There was no correlation between protein A and any of the 26 antigenic characteristics which have been previously described in these strains.
机译:可以使用放射性标记的骨髓瘤球蛋白在完整细菌的细胞壁上检测到金黄色葡萄球菌的蛋白A。 156个 S菌株。金黄色葡萄球菌中有141个(90%)含有蛋白质A。47个 S中没有一个。表皮葡萄球菌菌株的蛋白A呈阳性。蛋白A的产生受培养温度的影响,但不受培养时间或接种量大小的影响。含有高浓度NaCl的培养基可将蛋白A的产生抑制90%。用福尔马林处理含蛋白A的菌株导致检测量减少,但在4 C储存后未检测到进一步降低。在蛋白A的存在与否与噬菌体类型或噬菌体组之间未发现相关性。十六个 S。对金黄色葡萄球菌进行了广泛的研究。在这些菌株中,蛋白A与先前已描述的26种抗原特性之间没有相关性。

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