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首页> 外文期刊>Infection and immunity >Hemodialysis Culture of Serratia marcescens in a Goat-Artificial Kidney-Fermentor System
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Hemodialysis Culture of Serratia marcescens in a Goat-Artificial Kidney-Fermentor System

机译：山羊-人工肾脏-发酵罐系统中粘质沙雷氏菌的血液透析培养

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Hemodialysis was employed to simulate in vivo conditions for growth in mammalian blood, but without phagocytosis, by using the goat and Serratia marcescens as a host-parasite model. The blood stream was shunted surgically via prosthetic tubing from a carotid artery through the hollow-fiber membranes in an artificial kidney hemodialyzer and back into a jugular vein. The dialysate solution concurrently was pumped from a modular fermentor through the hemodialyzer jacket outside of the membranes and back into the fermentor. Hemodialysis between the two circuits was maintained continuously. When equilibrium was attained, bacteria inoculated into the dialysate circuit multiplied first exponentially at the maximal rate and then arithmetically at a lesser rate equally well under aerobic or anaerobic conditions. When a population of about 109 viable bacteria/ml was exceeded, the goat reacted acutely with signs of general toxemia, pyrexia, and leukopenia, apparently because of dialyzable toxic material produced by the culture. The maximal molecular size of the toxic material was defined relative to a rigid globular protein of 15,000 in molecular weight and 1.9 nm in hydrodynamic radius or to a flexible fibrous polyglycol of 5,500 in molecular weight and 2.6 nm in hydrodynamic radius, based on determinations of the membrane porosity threshold for dialysis.
机译：通过使用山羊和粘质沙雷氏菌作为宿主-寄生虫模型，通过血液透析来模拟体内血液在哺乳动物血液中生长但无吞噬作用的条件。血流通过人工管从颈动脉通过人造肾脏血液透析仪中的中空纤维膜分流，然后返回颈静脉。同时将透析液从模块化发酵罐中泵出，穿过膜外部的血液透析器夹套，再送回发酵罐中。连续维持两个回路之间的血液透析。当达到平衡时，在有氧或无氧条件下，接种到透析液回路中的细菌首先以最大速率成倍增长，然后以算术较低的速率成倍增长。当超过每毫升10 9 活菌的种群时，山羊发生急性反应，伴有一般的毒血症，发热和白细胞减少的迹象，这显然是由于培养物产生了可透析的有毒物质。相对于分子量为15,000且流体动力学半径为1.9 nm的刚性球状蛋白，或分子量为5,500且流体动力学半径为2.6 nm的柔性纤维状聚乙二醇，根据有毒物质的测定，确定了有毒物质的最大分子大小。透析膜的孔隙率阈值。

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