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首页> 外文期刊>Infection and immunity >Antibody response in the parotid fluid and serum of Irus monkeys (Macaca fascicularis) after local immunization with Streptococcus mutans.
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Antibody response in the parotid fluid and serum of Irus monkeys (Macaca fascicularis) after local immunization with Streptococcus mutans.

机译：变形链球菌局部免疫后，Irus猴（猕猴）的腮腺液和血清中的抗体反应。

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The antibody response of Macaca fascicularis in parotid saliva and serum to local immunization by two routes with Streptococcus mutans was studied and compared over 1 year. Antibodies were titrated and classified by indirect immunofluorescent staining using specific antiglobulin conjugates. Antiglucosyltransferase activity was assayed by an enzyme inhibition test. Animals were immunized first by injecting formalin-killed bacterial cells and cell products subcutaneously into the vicinity of the four major salivary glands. The monkeys were next immunized by retrograde instillation of antigen into the parotid duct. Extensive subcutaneous local immunization gave a serum response only. After parotid duct immunization, high titers of immunoglobulin A (IgA) antibody, along with traces of immunoglobulin G (IgG) immunoglobulin M (IgM) antibody, appeared in the parotid saliva, and in the serum high titers of IgG antibody were present along with lower titers of IgA and IgM. IgA antibodies in parotid fluid were shown by double immunofluorescent staining to be associated with antigenic determinants which cross-reacted with an antiserum directed to human secretory component. Titers in parotid fluids and sera fell sharply when immunization was stopped. This response pattern was reproducible. High concentrations of antibody capable of inhibiting glucosyltransferase prepared from S. mutans were found in the sera, but relatively little was detected in the parotid fluids. Extensive immunization via the parotid duct resulted in transient functional impairment of the gland, as evidenced by diminished salivary flow rates. We conclude that parotid ductal immunization can be an effective method for stimulating a salivary secretory IgA antibacterial antibody response.
机译：研究和比较了变形链球菌两种途径对腮腺唾液和血清中猕猴的局部免疫的抗体应答。使用特异性抗球蛋白偶联物通过间接免疫荧光染色对抗体进行滴定和分类。抗葡糖基转移酶活性通过酶抑制试验测定。首先通过皮下注射福尔马林杀死的细菌细胞和细胞产物到四个主要唾液腺附近，对动物进行免疫。接下来，通过将抗原逆行滴入腮腺导管来免疫猴子。广泛的皮下局部免疫仅产生血清反应。腮腺导管免疫后，腮腺唾液中出现了高滴度的免疫球蛋白A（IgA）抗体以及微量的免疫球蛋白G（IgG）免疫球蛋白M（IgM）抗体，血清中还出现了高滴度的IgG抗体以及IgA和IgM的滴度较低。双重免疫荧光染色显示腮腺液中的IgA抗体与抗原决定簇有关，该抗原决定簇与针对人类分泌成分的抗血清发生交叉反应。停止免疫后腮腺液和血清中的滴度急剧下降。此响应模式是可重现的。在血清中发现高浓度的能够抑制由变形链球菌制备的葡糖基转移酶的抗体，但是在腮腺液中检测到的抗体相对较少。唾液流速降低证明了通过腮腺导管的广泛免疫导致短暂的腺体功能受损。我们得出结论，腮腺导管免疫可以是一种刺激唾液分泌型IgA抗菌抗体应答的有效方法。

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