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首页> 外文期刊>Infection and immunity >Antigenic analysis of isolated polypeptides from Visna virus.
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Antigenic analysis of isolated polypeptides from Visna virus.

机译：从维斯纳病毒分离的多肽的抗原分析。

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The antigenic activity of 10 Visna polypeptides separated by gel filtration in the presence of 6 M guanidine hydrochloride (GuHCl) was examined with rabbit antisera made specific for Visna virus. The results showed that the first (GuHCl 1) and the ninth (GuHCl 9) polypeptide peak reacted with the antisera when examined in immunodiffusion, passive hemagglutination, and complement fixation tests. Whole virus, GuHCl 1, and GuHCl 9, when tested with the antisera, appeared to be immunologically identical in the immunodiffusion test. However, GuHCl 1 reacted weakly with the antisera by all three techniques as compared with GuHCl 9 and whole virus. GuHCl 9, when subjected to polyacrylamide gel electrophoresis containing 0.1% sodium lauryl sulfate, revealed the presence of one polypeptide with a molecular weight of 25,000. By the same method, GuHCl 1 was found to contain an aggregate of four different polypeptides, the major one having a molecular weight of 25,000. The results indicate that the antigenic activity of both GuHCl 1 and GuHCl 9 was associated with a single polypeptide having a molecular weight of 25,000.
机译：用对Visna病毒具有特异性的兔抗血清检查了在6 M盐酸胍（GuHCl）存在下通过凝胶过滤分离的10株Visna多肽的抗原活性。结果显示，在免疫扩散，被动血凝和补体固定试验中检查时，第一个（GuHCl 1）和第九个（GuHCl 9）多肽峰与抗血清反应。用抗血清测试时，全病毒GuHCl 1和GuHCl 9在免疫扩散测试中似乎在免疫学上是相同的。然而，与GuHCl 9和整个病毒相比，通过所有三种技术，GuHCl 1与抗血清反应较弱。当对GuHCl 9进行含有0.1％月桂基硫酸钠的聚丙烯酰胺凝胶电泳时，发现存在一种分子量为25,000的多肽。通过相同的方法，发现GuHCl 1包含四种不同多肽的聚集体，主要的一种具有25,000的分子量。结果表明，GuHCl 1和GuHCl 9的抗原活性均与分子量为25,000的单个多肽有关。

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