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首页> 外文期刊>Infection and immunity >Purification and partial characterization of hepatitis e antigen (HBeAg).
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Purification and partial characterization of hepatitis e antigen (HBeAg).

机译：戊型肝炎抗原（HBeAg）的纯化和部分表征。

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Purification of hepatitis e antigen (HBeAg) from 200 ml of chimpanzee plasma was accomplished by a combination of ion-exchange chromatography on diethylaminoethyl-cellulose followed by gel filtration. High-resolution sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified HBeAg demonstrated two major polypeptides with estimated molecular weights of 22,000 and 55,000. HBeAg labeled with 125I showed a high affinity for protein A-conjugated Sepharose CL-4B. The precipitation reaction between HBeAg and anti-HBe was inhibited by preincubating the purified antigen with rabbit anti-human immunoglobulin G (IgG). These data show that HBeAg is associated with a serum fraction with the biophysical and antigenic properties of an immunoblobulin of the IgG class. Sedimentation coefficient analysis of purified HbeAg resulted in an S20w value of 11.6 and a molecular weight value of 324,000. These findings, supported by gel filitration and polyacrylamide gradient gel electrophoresis, revealed that HBeAg has properties analogous to those of a dimer of IgG.
机译：通过在二乙氨基乙基纤维素上进行离子交换色谱，然后进行凝胶过滤的组合，从200 ml黑猩猩血浆中纯化e型肝炎抗原（HBeAg）。纯化的HBeAg的高分辨率十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示出两种主要多肽，估计分子量分别为22,000和55,000。用125 I标记的HBeAg对结合​​蛋白A的Sepharose CL-4B具有很高的亲和力。通过将纯化的抗原与兔抗人免疫球蛋白G（IgG）预孵育，可抑制HBeAg与抗HBe之间的沉淀反应。这些数据表明，HBeAg与具有IgG类免疫球蛋白的生物物理和抗原特性的血清组分有关。纯化的HbeAg的沉降系数分析得出S20w值为11.6，分子量值为324,000。这些发现得到凝胶过滤和聚丙烯酰胺梯度凝胶电泳的支持，表明HBeAg具有类似于IgG二聚体的特性。

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