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Comparison of Assay of Coliform Enterotoxins by Conventional Techniques Versus In Vivo Intestinal Perfusion

机译：大肠菌群毒素常规方法与体内肠道灌肠方法的比较

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Thirty-six strains of coliform bacteria were tested for enterotoxigenicity both by conventional assays, including the Y-1 adrenal and Chinese hamster ovary cell assays for heat-labile toxin and the suckling mouse assay for heat-stable toxin, and by determining the ability of graded concentrations of ultrafiltrate high- or low-molecular-weight toxin preparations to induce water secretion during in vivo perfusion in the rat jejunum. The ultrafiltrates of all 18 strains isolated from persons with infectious diarrheal disease, including seven of Escherichia coli, seven of Klebsiella pneumoniae, and four of Enterobacter cloacae, contained one (nine strains) or two (nine strains) potent toxin fractions (resembling either heat-labile or heat-stable toxin in terms of apparent molecular weight and heat lability characteristics) that induced water secretion at perfusion concentrations of 10 ng/ml or less. Unconcentrated broth filtrates of five of the E. coli strains and two of Klebsiella reacted positively in one or more of the conventional assay systems. Concentrated ultrafiltrates from two strains that were negative in the in vitro assays for heat-labile toxin were tested and also proved to be inactive in these test systems. None of 18 strains isolated from control sources produced, in the ultrafiltrates, enterotoxins capable of inducing water secretion at low concentrations, and none reacted positively in the conventional assays. These results indicate that some strains of coliform bacteria elaborate potent toxin materials that are capable of inducing water secretion and can be detected by perfusion of concentrated ultrafiltrates but not by conventional assay systems for enterotoxigenicity. Whether this represents quantitative or qualitative differences between the toxin materials that stimulate these different test systems remains to be established.
机译：通过常规检测方法测试了36株大肠菌群的肠毒素生成能力，包括Y-1肾上腺和中国仓鼠卵巢细胞热不稳定毒素检测法和乳鼠热稳定毒素检测法，并通过测定分级浓度的超滤液高分子量或低分子量毒素制剂在大鼠空肠体内灌注过程中诱导水分泌。从传染性腹泻病患者中分离出的所有18种菌株的超滤液，包括7株大肠杆菌，7株肺炎克雷伯菌和7株阴沟肠杆菌。 >，包含一种（九个菌株）或两个（九个菌株）有效的毒素级分（就表观分子量和热不稳定性而言，类似于热不稳定或热稳定的毒素），它们在灌注浓度为10 ng /时诱导水分泌。毫升或更少。五种 E的未浓缩肉汤滤液。一株或多株常规检测系统中，大肠埃希菌菌株和两种克雷伯菌阳性反应。测试了来自两种菌株的浓缩超滤液，这些菌株在体外对热不稳定毒素的测定中呈阴性，并且在这些测试系统中也被证明是无活性的。从对照源中分离出的18株菌株中，没有一种在超滤液中产生能够以低浓度诱导水分泌的肠毒素，并且在常规测定中没有阳性反应。这些结果表明，一些大肠菌群菌株精心制作了有效的毒素物质，这些物质能够诱导水分分泌，并且可以通过灌注浓缩的超滤液来检测，但不能通过常规的产肠毒素分析系统进行检测。这是否代表刺激这些不同测试系统的毒素材料之间的数量或质量差异尚待确定。

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