...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Anaerobic Bacteria on the Mucosal Epithelium of the Murine Large Bowel
【24h】

Anaerobic Bacteria on the Mucosal Epithelium of the Murine Large Bowel

机译：小鼠大肠粘膜上皮细胞上的厌氧细菌

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Anaerobic bacteria can be detected at population levels of 1011 organisms per g of cecum or colon in adult mice from four different colonies widely spaced in the United States. Most of these microorganisms are oxygen-intolerant fusiform-shaped bacteria. At least one type of these tapered, rod-shaped bacteria can be seen in layers in the epithelial mucin in frozen-section histological preparations of the large bowels of mice. In addition, such microorganisms can be seen within 0.5 μm of the epithelium in ultrathin sections of colon or cecum examined in an electron microscope. These fusiform-shaped bacteria predominate in the mucin layers. However, spiral-shaped microorganisms can be found as well near the mucosal epithelia in ultrathin sections of colon. Also, such organisms can be seen in negatively-stained preparations of washings of the colonic mucosal epithelia examined in an electron microscope. At least three types of spiral-shaped organisms, including both spiral-shaped bacteria and spirochetes, can be found in preparations from mice from three of the four colonies. Such spiral-shaped microorganisms can be detected at population levels as great as 109 organisms per g of cecum or colon in anaerobic cultures of the large bowels of mice from all four colonies. One anaerobic spiral bacterium was isolated in pure culture. This particular organism was found by immunofluorescence to be intermingled with the fusiform-shaped bacteria in the mucin on the mucosal epithelium in the mouse large bowel.
机译：在美国分布较广的四个不同菌落的成年小鼠中，每克盲肠或结肠中的10 11 生物体的种群水平可检测到厌氧细菌。这些微生物大多数是不耐氧的梭形细菌。在小鼠大肠的冷冻切片组织学制剂中，在上皮粘蛋白的层中可以看到至少一种类型的这些锥形杆状细菌。另外，在电子显微镜检查的结肠或盲肠的超薄切片中，可以在上皮的0.5μm以内看到这种微生物。这些梭形细菌在粘蛋白层中占主导。但是，在结肠超薄部分的粘膜上皮附近也可以发现螺旋状微生物。同样，在电子显微镜下检查的结肠粘膜上皮清洗液的阴性染色制剂中也可以看到这种生物。在来自四个菌落中的三个菌落的小鼠的制剂中，至少可以发现三种类型的螺旋形生物，包括螺旋形细菌和螺旋体。在来自所有四个菌落的小鼠大肠的厌氧培养物中，每克盲肠或结肠中可以检测到多达10 9 个细菌的螺旋形微生物。在纯培养物中分离出一种厌氧螺旋细菌。通过免疫荧光发现该特定生物与小鼠大肠粘膜上皮粘液中的梭形细菌混合。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号