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Three Characteristics Associated with Enterotoxigenic Escherichia coli Isolated from Man

机译:分离自人的产肠毒素大肠埃希菌的三个特征

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In Dacca, Bangladesh, potent enterotoxin-producing Escherichia coli were isolated from many hospital cases of acute cholera-like diarrhea. Enterotoxigenic (tox+) and non-enterotoxigenic (tox?) isolates of E. coli were used to investigate possible means of differentiating tox+E. coli from those (tox?) of the normal flora. The majority (81%) of the tox+E. coli studied were found to be negative for sucrose fermentation, 85% exhibited retarded growth in a peptone medium at pH 8.5, and 92% released large amounts of ammonium sulfate precipitable materials into culture supernatant fluids; 66.6% exhibited all three of these properties. For the tox? group the respective values were found to be 50%, 31%, and 34%; only 9.3% exhibited all three properties. These results indicate that it may be possible to use phenotypic characteristics other than antigenic composition and enterotoxin production for the identification of enterotoxigenic E. coli.
机译:在孟加拉国达卡,从许多急性霍乱样腹泻医院病例中分离出了有效的产肠毒素的大肠杆菌。 E的肠毒素(tox + )和非肠毒素(tox ?)分离株。大肠杆菌被用来研究区分tox + E的可能方法。大肠杆菌(来自正常菌群的毒素(tox ?))。 tox + E的大部分(81%)。发现所研究的大肠杆菌对蔗糖发酵不利,在pH 8.5的蛋白ept培养基中,有85%的蛋白质显示出生长缓慢,并且有92%的蛋白质将大量的硫酸铵可沉淀物质释放到培养上清液中。 66.6%的产品具有这三个特性。对于tox ?组,发现各自的值分别为50%,31%和34%;只有9.3%的产品具有这三种性能。这些结果表明,可以使用抗原成分和肠毒素产生以外的表型特征来鉴定产肠毒素的 E。大肠杆菌。

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