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首页> 外文期刊>Infection and immunity >Immunoelectrophoretic Analysis of Mycoplasma mycoides var. mycoides
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Immunoelectrophoretic Analysis of Mycoplasma mycoides var. mycoides

机译：支原体变种的免疫电泳分析杀菌剂

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Acrylamide gel electrophoresis was used to show the similarities and differences in the membrane proteins of two vaccine and two virulent strains of Mycoplasma mycoides var. mycoides. Immunoelectrophoretic (IEP) analysis was also used to partially characterize the associated antigens. Antibody spectra to the antigens of M. mycoides differ in rabbit, pig, and cattle sera. Rabbits produce better precipitating antibody against the anodic migrating protein mycoplasma antigens than cattle and pigs as seen in IEP. However, rabbit anti-M. mycoides serum did not show precipitating antibody against the heat-stable carbohydrate antigen. As judged by IEP, the major carbohydrate antigen extracted from the media, or boiled whole organism, is similar to that present in the sera-infected cattle and knee joints of calves. This carbohydrate antigen has a cathodic migration in IEP at pH 8.6. Periodate oxidation, classically used to destroy carbohydrate, also destroys most of the protein antigens. Heating the antigens to 56 C for 10 min destroys many of the noncarbohydrate antigens and 100 C eliminates all but the carbohydrate antigen. Extraction of M. mycoides with chloroform-methanol, phenol, ethanol, or ethanol-acetone reduced or eliminated most of the protein antigens. Some of the isolated antigenic fractions of M. mycoides were tested to determine their activity in the diagnostic complement fixation test for contagious bovine pleuropneumonia and their inhibitory effect in this test by using bovine anti-M. mycoides antisera having precipitating antibody and circulating antigen. The complement fixation antigen is not the galactan, cannot be extracted by chloroform-methanol, but is stable to boiling at 100 C and may be extracted by phenol and partially precipitated by ethanol-acetone.
机译：用丙烯酰胺凝胶电泳显示了两种疫苗和两种强毒株 Mycoplasma mycoides var的膜蛋白的异同。 mycoides 。免疫电泳（IEP）分析也可用于部分鉴定相关抗原。 M抗原的抗体谱。杀菌剂在兔子，猪和牛的血清中有所不同。如在IEP中所见，兔子产生的抗阳极迁移蛋白支原体抗原的沉淀抗体比牛和猪更好。但是，兔抗-m。杀真菌剂血清未显示出针对热稳定碳水化合物抗原的沉淀抗体。根据IEP的判断，从培养基或煮沸的整个生物中提取的主要碳水化合物抗原与感染血清的牛和犊牛膝关节中的碳水化合物相似。该碳水化合物抗原在pH 8.6的IEP中具有阴极迁移。高碘酸盐氧化，通常用于破坏碳水化合物，也破坏大多数蛋白质抗原。将抗原加热至56 C 10分钟会破坏许多非碳水化合物抗原，而100 C则会消除除碳水化合物抗原以外的所有抗原。提取 M。杀菌剂与氯仿-甲醇，苯酚，乙醇或乙醇-丙酮可减少或消除大多数蛋白质抗原。一些 M的分离抗原部分。通过使用牛抗 M，测试了真菌毒素，以确定它们在诊断性补体固定试验中对牛传染性胸膜肺炎的活性以及在该试验中的抑制作用。具有沉淀抗体和循环抗原的抗真菌药抗血清。补体固定抗原不是半乳聚糖，不能用氯仿-甲醇提取，但在100℃下稳定沸腾，可以用苯酚提取，并用乙醇-丙酮部分沉淀。

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