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首页> 外文期刊>Infection and immunity >Effects of Lysozyme and Chitinase on the Spherules of Coccidioides immitis In Vitro
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Effects of Lysozyme and Chitinase on the Spherules of Coccidioides immitis In Vitro

机译：溶菌酶和几丁质酶对球虫球菌体外炎症球的影响

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Spherules of Coccidioides immitis strain Silveira produced in vitro were treated with chitinase and lysozyme. The walls of merthiolate-killed mature endosporulating spherules were degraded by chitinase (500 μg/ml) and by lysozyme (100 and 500 μg/ml). Thus, as was visible through the light microscope, the spherule wall was reduced in thickness from 1 to 2 μm to less than 0.5 μm. The degradation was evident also by release of N-acetylglucosamine, three times as much N-acetylglucosamine being released by chitinase in 12 h as was released by lysozyme in 3 days. However, the effect of lysozyme on living mature spherules was in marked contrast to the effect of chitinase in that treatment with lysozyme led to marked reduction in viability. Exposure to lysozyme (500 μg/ml) for 48 h permitted survival of only 0 to 0.2% of spherules. Thinning of the walls was observed only in the larger spherules (25-35 μm) treated with lysozyme. By contrast, chitinase (500 μg/ml) led to complete dissolution of the walls of living mature spherules but the viability of the liberated endospores was unaffected during contact with chitinase for 48 h. Living non-endosporulating immature spherules and free endospores were also rendered nonviable by lysozyme but not by chitinase.
机译：用几丁质酶和溶菌酶处理体外产生的 Coccidioides immitis 菌株Silveira的球。被甲硫醇盐杀死的成熟内孢子球的壁被几丁质酶（500μg/ ml）和溶菌酶（100和500μg/ ml）降解。因此，如通过光学显微镜可见的，小球壁的厚度从1-2μm减小至小于0.5μm。降解也通过 N -乙酰氨基葡萄糖的释放而明显，几丁质酶在12小时内释放的 N -乙酰氨基葡萄糖的量是溶菌酶在3天内释放的三倍。然而，溶菌酶对活的成熟小球的作用与几丁质酶的作用形成鲜明对比，因为用溶菌酶处理会导致活力显着降低。暴露于溶菌酶（500μg/ ml）48小时只能使小球的存活率为0％至0.2％。仅在溶菌酶处理过的较大球体（25-35μm）中观察到壁变薄。相比之下，几丁质酶（500μg/ ml）导致活的成熟小球壁完全溶解，但与几丁质酶接触48 h时，释放的内生孢子的活力并未受到影响。还通过溶菌酶而不是几丁质酶使活的非内生孢子的未成熟球体和游离的内生孢子不能存活。

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