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Enhancement of Human Neutrophil Bactericidal Activity by Chemotactic Factors

机译：趋化因子增强人类嗜中性粒细胞的杀菌活性

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Neutrophils are important effector cells in the defense against microorganisms. They migrate into infected sites and then phagocytose and kill bacteria. Chemotactic factors may be important for initiating neutrophil migration. We investigated whether chemotactic factors might also influence an event subsequent to chemotaxis, namely bacterial killing. It was found that preincubation (20 min at 37°C) of human leukocytes with chemotactic substances such as zymosan-activated serum, a C5a-containing fraction of zymosan-activated serum, N-formyl methionyl phenylalanine or N-formyl methionyl-leucine-phenylalanine, enhanced leukocyte killing of Staphylococcus aureus, Escherichia coli, and Streptococcus faecalis in a dose-dependent fashion. The concentration of chemotactic factor required to enhance killing was similar to that required to induce neutrophil chemotaxis. In addition, zymosan-activated serum, C5a fraction, and the two N-formyl methionyl peptides increased the hexose monophosphate shunt activity of resting and phagocytosing neutrophils by two- to threefold. In contrast, bacterial killing by sodium azide-treated neutrophils and neutrophils from a patient with chronic granulomatous disease was not increased by any chemotactic factor. These findings suggest that chemotactic factors stimulate neutrophil oxygen-dependent microbicidal pathways. These observations illustrate another important contribution of biologically active molecules to effector cell function and host defense.
机译：中性粒细胞是防御微生物的重要效应细胞。它们迁移到感染部位，然后被吞噬并杀死细菌。趋化因子对于启动中性粒细胞迁移可能很重要。我们调查了趋化因子是否也可能影响趋化后的事件，即细菌杀灭。发现将人白细胞与趋化性物质（如酵母聚糖激活的血清，含有C5a的酵母聚糖激活的血清， N -甲酰基甲硫基苯丙氨酸或 N -甲酰基甲硫氨酸-亮氨酸-苯丙氨酸，增强了金黄色葡萄球菌，大肠杆菌和粪链球菌的白细胞杀伤作用，且呈剂量依赖性。增强杀伤作用所需的趋化因子浓度与诱导中性粒细胞趋化作用所需的浓度相似。此外，酵母聚糖激活的血清，C5a馏分和两个 N -甲酰甲硫氨酰肽使静止和吞噬嗜中性粒细胞的己糖一磷酸分流活性增加了两到三倍。相比之下，叠氮化钠处理的嗜中性粒细胞和慢性肉芽肿病患者的嗜中性粒细胞对细菌的杀灭没有增加任何趋化因子。这些发现表明趋化因子刺激中性粒细胞氧依赖性杀微生物途径。这些观察结果说明了生物活性分子对效应细胞功能和宿主防御的另一重要贡献。

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