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Comparative toxic effect of the surface lipid of Corynebacterium ovis on peritoneal macrophages.

机译：棒状杆菌的表面脂质对腹膜巨噬细胞的比较毒性作用。

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摘要

The postphagocytic effect on mouse, rabbit, and guinea pig peritoneal macrophages of a petrol-ether lipid extract from Corynebacterium ovis (C. pseudotuberculosis) representing the surface coat of the organism external to the cell wall was investigated by examing three parameters of cytotoxicity, viability assayed by dye exclusion, glycolytic activity, and ultrastructural morphology. The viability test demonstrated a lethal effect on normal and immune mouse macrophages but not on those of the rabbit or guinea pig. Measurement of glycolsis indicated a significant degree of cytotoxicity in normal mouse macrophages ingesting lipid, a nonsignificant depression of activity in cells from immune mice, and no alteration in the activities of rabbit and guinea pig macrophages. Electron microscopy demonstrated that C. ovis surface lipid caused acute lethal injury in normal and immune mouse macrophages. The early stages of degeneration were typified by dilatation of the cisternae of rough endoplasmic reticulum, Golgi lamellae, and nuclear envelope, proceeding to focal disruption of various cell membranes, particularly those of the lipidcontaining phagolysosomes and nucleus. In contrast, over the 3-h period of study, no cytotoxic change was evident in rabbit or guinea pig macrophages. The results add further support to previous observations that the surface lipid of C. ovis plays a major role in the pathogenesis of the organism in mice, but they do not explain the guinea pig's marked susceptibility to infection.
机译：通过检查细胞毒性，存活力的三个参数，研究了代表动物壁外部生物的棒状棒杆菌（假结核杆菌）的汽油醚脂质提取物对小鼠，兔子和豚鼠腹膜巨噬细胞的吞噬后作用。通过染料排斥，糖酵解活性和超微结构形态进行分析。生存力测试显示对正常和免疫小鼠巨噬细胞具有致死作用，但对兔子或豚鼠却没有。糖酵解的测量表明摄取脂质的正常小鼠巨噬细胞具有显着程度的细胞毒性，免疫小鼠细胞的活性无明显降低，兔子和豚鼠巨噬细胞的活性没有改变。电子显微镜显示，C。ovis表面脂质对正常和免疫小鼠巨噬细胞造成急性致死性伤害。变性的早期以粗糙的内质网，高尔基片状细胞和核被膜的水箱扩张为代表，并逐渐破坏各种细胞膜，特别是含有脂质的吞噬溶酶体和细胞核。相反，在研究的3小时内，在兔子或豚鼠巨噬细胞中没有明显的细胞毒性变化。该结果为以前的观察结果提供了进一步的支持，即观察到C. ovis的表面脂质在小鼠有机体的发病机理中起着重要作用，但它们不能解释豚鼠对感染的明显易感性。

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