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首页> 外文期刊>Infection and immunity >Morphology and viability of large bodies of streptococcal L-forms.
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Morphology and viability of large bodies of streptococcal L-forms.

机译：链球菌L型大体的形态和生存力。

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A procedure is described for the massive formation and isolation of large bodies of group A and D streptococcal L-forms. Up to 90% individual bodies of 20 to 100 mum in diameter can be produced in pour plates of nutrient gelatin and subsequently harvested by simple micromanipulation. The growth of these giant bodies was followed by light microscopy and their ultrastructure and internal architecture was examined by transmission and scanning electron microscopy. Large bodies had a honeycomb structure of vacuoles. Numerous small units could be observed inside, protruding into, and between vacuoles. Intact large bodies (30 to 60 mum), when placed in small amounts of broth and incubated at 35 C, initiated turbid cultures, but when set on agar, they ruptured, releasing internal granules and producing typical L-form colonies. The number of internal colony-forming units (CFU) was correlated with the size of large bodies. Up to 200 CFU were detected in bodies of 40 to 60 mum in diameter, whereas corpuscles of 20 to 30 mum averaged only three CFU. The inefficiency of replication was apparent with the determination by light and electron microscopy that at least 100 times as many granules and elementary corpuscles as CFU were produced inside large bodies.
机译：描述了大规模形成和分离A和D组链球菌L型大体的程序。在营养明胶倾倒板中最多可产生90％的直径为20至100微米的单个物体，然后通过简单的显微操作将其收获。通过光学显微镜观察这些巨型体的生长，并通过透射电子显微镜和扫描电子显微镜检查其超微结构和内部结构。大物体具有液泡的蜂窝状结构。在内部，突入液泡中和液泡之间可以观察到许多小的单元。完整的大体（30至60毫米），当放入少量肉汤中并在35°C孵育时，会开始混浊培养，但是当置于琼脂上时，它们会破裂，释放内部颗粒并产生典型的L型菌落。内部菌落形成单位（CFU）的数量与大物体的大小相关。在直径为40至60微米的尸体中检测到高达200 CFU，而20至30毫米的小体平均只有3个CFU。通过光镜和电子显微镜确定，复制的效率很低，即在大体内至少产生了100倍于CFU的颗粒和基本小体。

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