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首页> 外文期刊>Infection and immunity >Role of the Phagocyte in Host-Parasite Interactions XXIV. Aldehyde Generation by the Myeloperoxidase-H2O2-Chloride Antimicrobial System: a Possible In Vivo Mechanism of Action
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Role of the Phagocyte in Host-Parasite Interactions XXIV. Aldehyde Generation by the Myeloperoxidase-H2O2-Chloride Antimicrobial System: a Possible In Vivo Mechanism of Action

机译：吞噬细胞在宿主-寄生虫相互作用中的作用XXIV。髓过氧化物酶-H2O2-氯化物抗菌系统生成的醛：可能的体内作用机理。

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Myeloperoxidase (MPO), H2O2, and chloride ions in the presence of bacteria form aldehydes and are bactericidal. The use of heat-inactivated MPO prevented both killing and aldehyde generation. Decarboxylation and deamination of carboxyl and amino group substrates arising from the bacterial surface may participate in the reaction which yields aldehydes. Bacterial contact was essential for killing. Decarboxylation and bactericidal activities were noted when physiological concentrations of chloride were used. When MPO was replaced with horseradish peroxidase (HPO) in the chloride medium, decarboxylation and bactericidal activities were no longer noted. In contrast, iodide functioned in the antimicrobial system with either MPO or HPO. The iodide concentrations required were at least sixfold greater than circulating blood iodide levels. Moreover, decarboxylation did not occur in the presence of iodide with either enzyme. Thus, both halides function in the MPO-H2O2 system but by different mechanisms. It is likely that in vivo under most conditions chloride is the functional halide and that generation of aldehydes is the mechanism responsible for the antimicrobial activity of the MPO-H2O2-chloride system.
机译：细菌存在时，髓过氧化物酶（MPO），H 2 O 2 和氯离子形成醛并具有杀菌作用。使用热灭活的MPO可以防止杀灭和醛的产生。由细菌表面引起的羧基和氨基底物的脱羧和脱氨基可能参与反应，产生醛。细菌接触对于杀死细菌至关重要。当使用生理浓度的氯化物时，注意到脱羧和杀菌活性。当在氯化物培养基中用辣根过氧化物酶（HPO）代替MPO时，不再注意到脱羧和杀菌活性。相反，碘化物在MPO或HPO的抗菌系统中起作用。所需的碘化物浓度比循环血液中的碘化物浓度至少高六倍。而且，在碘化物存在下，两种酶都不会发生脱羧。因此，两种卤化物均在MPO-H 2 O 2 系统中起作用，但机制不同。在大多数情况下，体内氯可能是功能性卤化物，醛的产生可能是MPO-H 2 O 2 -的抗菌活性的机制。氯化物系统。

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