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首页> 外文期刊>Infection and immunity >Mechanism of Action of Intestinal Antibody in Experimental Cholera II. Antibody-Mediated Antibacterial Reaction at the Mucosal Surface
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Mechanism of Action of Intestinal Antibody in Experimental Cholera II. Antibody-Mediated Antibacterial Reaction at the Mucosal Surface

机译：肠道抗体在实验霍乱中的作用机理粘膜表面的抗体介导的抗菌反应

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Earlier studies have shown that intestinal antibody (coproantibody) decreases the adsorption of vibrios to the intestinal wall of rabbits. The mechanism underlying this phenomenon was studied by means of an in vitro model in which vibrios were grown on slices of rabbit ileum in a moist chamber. Antibody prevented the adsorption of vibrios onto such slices in the same manner as it did in vivo. Studies of the in vitro growth rate of vibrios on slices of ileum indicated an antibody-dependent antibacterial mechanism on the mucosal surface. This mechanism appeared to require the presence of viable mucosal cells, as it was not present when filter paper was substituted for the tissue slices and it could not be demonstrated in fresh scrapings from the intestinal mucosa. Antigen-antibody mixtures were added to the surface of slices of rabbit ileum or were introduced into the lumen of intestinal loops. Presence of these mixtures did not inhibit the antibody-dependent antibacterial mechanism on the mucosa in either the in vivo or the in vitro system. The amount of antigen-antibody mixture used in vivo was at least 12 times that required to neutralize the complement released by homogenization from an entire intestinal loop. The results obtained support the hypothesis that coproantibody protects by decreasing the adsorption of vibrios on the intestinal mucosa. The mechanism responsible appears to be an antibacterial effect on the mucosal surface which requires antibody plus some additional factor(s) supplied by viable mucosal cells. The postulated factor does not appear to be complement.
机译：较早的研究表明，肠道抗体（coproantibody）可降低弧菌对兔肠壁的吸附。通过体外模型研究了这一现象的机理，在体外模型中，弧菌在潮湿室内的兔回肠切片上生长。抗体以与体内相同的方式防止弧菌吸附到此类切片上。对回肠切片上弧菌的体外生长速率的研究表明，在粘膜表面存在抗体依赖性抗菌机制。该机制似乎需要存在活的粘膜细胞，因为当用滤纸代替组织切片时不存在这种机制，并且在从肠粘膜的新鲜刮屑中无法证明这一机制。将抗原-抗体混合物添加到兔回肠切片的表面或引入肠loop腔。这些混合物的存在在体内或体外系统中均不抑制粘膜上的抗体依赖性抗菌机制。体内使用的抗原-抗体混合物的量是中和从整个肠环匀浆化释放的补体所需量的至少12倍。获得的结果支持了共原抗体通过减少弧菌在肠粘膜上的吸附来保护的假说。负责的机制似乎是对粘膜表面的抗菌作用，这需要抗体加上活的粘膜细胞提供的一些附加因子。假定的因素似乎不是互补的。

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