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首页> 外文期刊>Infection and immunity >Purification and immunochemical characterization of type e polysaccharide antigen of Streptococcus mutans.
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Purification and immunochemical characterization of type e polysaccharide antigen of Streptococcus mutans.

机译：变形链球菌e型多糖抗原的纯化和免疫化学表征。

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The type-specific antigen of Streptococcus mutans strain MT703, serotype e, has been chromatographically purified and characterized. Two chromatographic fractions were obtained from saline extracts which reacted with both anti-MT703 whole-cell serum and Lancefield group E serum. The major fraction (eI) was identified as a polysaccharide composed of 37% glucose, 56% rhamnose, 5% protein, and 0.3% phosphorus, whereas the minor fraction (eII) contained 66% protein in addition to 10% glucose and 17% rhamnose. The immunological specificity of these antigens was found to be the same by immunodiffusion in agar gel. Another fraction with a negative charge (eIII) reacted with polyglycerophosphate antisera from Streptococcus mutans and Streptococcus pyogenes. For comparison, the MT703 antigen in a hot trichloroacetic acid extract (eA) and the group E antigen from a saline extract of cells of strain K129 (EI) were similarly purified by anionic ion-exchange chromatography. Although the ratio of glucose and rhamnose in eA was 1:0.9 and in eI and eII approximately 1:1.5, reactions of identity were obtained in gel diffusion against specific anti-e serum. This difference in ratio is probably a result of the extraction procedures. Both the type e and group E antisera were reactive with both eI and EI antigens. The adsorption of group E antiserum with MT703 cells removed all E antibody, whereas type e-specific antibody remained after adsorption with K129 cells. These results suggest that eI antigen possesses both e and E specificities, whereas EI possesses E only. These findings were supported by the quantitative precipitin test and immunodiffusion and/or immunoelectrophoretic patterns in agar gel. Methyl-beta-D-glucopyranoside markedly inhibited the precipitin reaction in both type e and group E sera. However, a significantly stronger inhibition by cellobiose of type e serum than of group E serum indicates that a beta-linked glucose-glucose dimer is the predominant antigenic determinant of the e specificity. The presence of both e and E specificities on a single polysaccharide molecule was demonstrated by the use of purified e antigen released from a specific e-anti-e complex. This antigen reacted with a group E-specific serum as well as a type e-specific serum. An examination of five S. mutans type e strains showed the presence of group E specificity also, whereas the I, II, and IV serotypes of group E streptococci only possessed the group E specificity.
机译：变形链球菌MT703株的血清型e的类型特异性抗原已进行色谱纯化和鉴定。从盐水提取物中获得两个色谱级分，其与抗MT703全细胞血清和Lancefield E组血清反应。主要部分（eI）被鉴定为由37％葡萄糖，56％鼠李糖，5％蛋白质和0.3％磷组成的多糖，而次要部分（eII）除10％葡萄糖和17％葡萄糖外还包含66％蛋白质。鼠李糖。通过琼脂凝胶中的免疫扩散发现这些抗原的免疫学特异性是相同的。带有负电荷的另一部分（eIII）与变形链球菌和化脓性链球菌的聚甘油磷酸抗血清反应。为了比较，通过阴离子交换色谱法类似地纯化热三氯乙酸提取物（eA）中的MT703抗原和菌株K129（EI）的细胞的盐水提取物中的E组抗原。尽管eA中葡萄糖和鼠李糖的比例为1：0.9，eI和eII中的比例约为1：1.5，但在针对特定抗e血清的凝胶扩散中获得了相同的反应。比率的这种差异可能是提取程序的结果。 e型和E组抗血清均与eI和EI抗原都有反应。 E组抗血清在MT703细胞上的吸附去除了所有E抗体，而E型特异性抗体在被K129细胞吸附后仍然保留。这些结果表明，eI抗原同时具有e和E特异性，而EI仅具有E。这些发现得到琼脂凝胶中定量沉淀蛋白试验和免疫扩散和/或免疫电泳模式的支持。甲基-β-D-吡喃葡萄糖苷在e型和E组血清中均显着抑制沉淀蛋白反应。然而，纤维二糖对E型血清的抑制作用明显强于E组血清，这表明β联结的葡萄糖-葡萄糖二聚体是e特异性的主要抗原决定因素。通过使用从特定的e-抗e-复合物释放的纯化的e抗原证明了单个多糖分子上e和E特异性的存在。该抗原与E族特异性血清以及E型特异性血清反应。对五种变形链球菌e型菌株的检查还显示了E组特异性，而E组链球菌的I，II和IV血清型仅具有E组特异性。

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