• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Antigenic Modification, Rosette-Forming Cells, and Salmonella typhimurium Resistance in Outbred and Inbred Mice
【24h】

Antigenic Modification, Rosette-Forming Cells, and Salmonella typhimurium Resistance in Outbred and Inbred Mice

机译：近亲和近交小鼠的抗原修饰，形成玫瑰花结的细胞和鼠伤寒沙门氏菌。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To assess the separate contributions of host T cells and the physical state of the antigen in the development of effective. Salmonella resistance, glutaraldehyde-treated and untreated protein- and ribonucleic acid-rich extracts (E-RNA extracts) of virulent Salmonella typhimurium SR-11 or attenuated S. typhimurium RIA were used to immunize Salmonella-resistant Salmonella-susceptible strains of mice for the purpose of determining whether antigen-specific T-cell or B-cell responses were formed and, if so, which responses predominated. The resistance imparted to each mouse strain after vaccination with S. typhimurium RIA was used as the standard for comparison. The inbred mouse strains C57BL/6 and DBA/2 and their F1 hybrid (strain BDF1), outbred ICR Swiss mice, and endotoxin-resistant C3H/HeJ mice were examined for the capacity to develop resistance to lethal Salmonella infections, as well as the ability to generate antigen-reactive T cells. Only the BDF1, C3H/HeJ, and ICR Swiss mice were able to develop resistance to challenge infections mediated by the virulent SR-11 strain of S. typhimurium after vaccination with the living, attenuated RIA strain of S. typhimurium or immunization with E-RNA extracts. We developed an assay to identify the antigen-reactive rosette-forming lymphocytes present in lymph nodes and spleens of immunized mice. Levels of 0.2% or higher of theta antigen-bearing, antigen-reactive rosette-forming cells were found in the lymph nodes or spleens or both of only the BDF1, C3H/HeJ, and ICR Swiss mice (i.e., in the “Salmonella responder” strains). Mouse strains C57BL/6 and DBA/2, which failed to develop resistance to lethal infections after immunization with the S. typhimurium RIA vaccine or with the E-RNA extracts, lacked effective numbers of antitheta antigen-sensitive rosette-forming cells. Modification of the effective E-RNA extracts by polymerization with glutaraldehyde resulted in a marked diminution in their abilities to induce resistance to salmonellosis in the two responder mouse strains tested (BDF1 and ICR Swiss), even though detectable levels of antibody were induced.
机译：对评估宿主T细胞的单独贡献和抗原在发展中的物理状态有效。耐沙门氏菌，戊二醛处理过的和未经处理的强毒鼠伤寒沙门氏菌 SR-11或减毒的 S蛋白质和核糖核酸富集提取物（E-RNA提取物）。鼠伤寒杆菌 RIA用于免疫耐沙门氏菌 Salmonella 的小鼠品系，以确定抗原特异性T细胞还是B细胞反应形成了，如果是这样的话，哪些回应占主导。接种 S后赋予每个小鼠品系的抗性。以鼠伤寒 RIA作为比较标准。自交系小鼠C57BL / 6和DBA / 2及其F 1 杂种（BDF 1 系），近交ICR Swiss小鼠和耐内毒素的C3H / HeJ小鼠检查了对致命性沙门氏菌感染的抵抗力，以及产生抗原反应性T细胞的能力。只有BDF 1 ，C3H / HeJ和ICR Swiss小鼠能够对由强毒SR-11菌株 S介导的攻击感染产生抵抗力。活的减毒的RIA株 S接种疫苗后，鼠伤寒沙门氏菌。鼠伤寒或用E-RNA提取物免疫。我们开发了一种检测方法，以鉴定存在于免疫小鼠淋巴结和脾脏中的抗原反应性玫瑰花结形成淋巴细胞。仅在BDF 1 ，C3H / HeJ和ICR Swiss小鼠的淋巴结或脾脏或两者中发现0.2％或更高的含theta抗原，抗原反应性玫瑰花结形成细胞的水平（即“沙门氏菌”响应株）。小鼠品系C57BL / 6和DBA / 2，在用 S免疫后未能产生对致命感染的抗性。鼠伤寒沙门氏菌RIA疫苗或E-RNA提取物缺少有效数量的抗θ抗原敏感玫瑰花结形成细胞。通过戊二醛聚合反应对有效E-RNA提取物的修饰导致在测试的两种应答小鼠品系（BDF 1 和ICR Swiss）中诱导沙门氏菌抗性的能力显着降低，即使可以检测到诱导抗体水平。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号