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首页> 外文期刊>Infection and immunity >Identification of the active precipitin components in a purified preparation of the A antigen of Blastomyces dermatitidis.
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Identification of the active precipitin components in a purified preparation of the A antigen of Blastomyces dermatitidis.

机译：在皮肤芽孢杆菌的A抗原的纯化制剂中鉴定活性沉淀蛋白成分。

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摘要

A purified A-antigen preparation of Blastomyces dermatitidis was determined to be composed of five major glycoprotein bands, visible with Coomassie blue and periodic acid-Schiff staining of polyacrylamide gels. At least 20 additional protein bands were detected by using a silver stain, which was 100 times more sensitive than the Coomassie method. Two components of this mixture were determined to be associated with the A-antigenic activity of B. dermatitidis. Of several antigen preparations examined in Ouchterlony precipitation tests, those reactive with a reference anti-A antiserum contained the slowest moving of the Coomassie blue bands. The antigen preparations without precipitin reactivity lacked this protein band. Two protein bands were shown to disappear from an antigen preparation after incubation with an affinity gel linked to the reference anti-A serum. One of the bands was the slowest Coomassie blue band, and the other was a fast-migrating protein detectable only with the silver stain. Characterization of the components responsible for the A-antigenic activity has important applications in the production and standardization of serological reagents for the diagnosis of blastomycosis.
机译：经测定，纯化的皮肤芽孢杆菌的A抗原制剂由五个主要糖蛋白带组成，考马斯蓝和聚丙烯酰胺凝胶的高碘酸-希夫染色可见。使用银染至少检测到20条额外的蛋白条带，其灵敏度比考马斯方法高100倍。确定该混合物的两种成分与皮肤病双歧杆菌的A抗原活性有关。在Ouchterlony沉淀测试中检查的几种抗原制剂中，与参考抗A抗血清反应的抗原制剂中考马斯蓝带移动最慢。没有沉淀蛋白反应性的抗原制剂缺少该蛋白条带。与连接至参考抗A血清的亲和凝胶孵育后，抗原制剂中显示出两条蛋白带消失。一条带是最慢的考马斯蓝带，另一条带是仅用银染才能检测到的快速迁移的蛋白质。负责A抗原活性的成分的表征在用于诊断母发菌病的血清学试剂的生产和标准化中具有重要的应用。

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