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Persistence of Adenovirus 5 in Guinea Pigs

机译：腺病毒5在豚鼠中的持久性

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One intracardiac inoculation of adenovirus 5 in guinea pigs leads to virus persistence in different organs, viz., 5 days in lungs and liver, 14 days in blood and lymph nodes, and 56 days or more in the spleen. After cultivation of tissue cells for 1 week, virus was recovered from blood, lymph nodes, or spleen lymphocytes, but virus could be detected directly in cells only when organs were removed within 48 h of inoculation. To determine how the virus persisted in low concentrations and as a latent infection, spleens were primarily selected for study by three techniques: homogenization of spleens, suspended Maitland fragment cultures, and in vitro cultivation of spleen cells. The last procedure showed virus in fibroblast-like cells (probably macrophages or reticuloendothelial cells) for 56 days after infection of guinea pigs. With other methods, the virus was found only within the first 2 days after inoculation.
机译：在豚鼠中一次心内接种腺病毒5导致病毒在不同器官中持续存在，即在肺和肝中持续5天，在血液和淋巴结中持续14天，在脾脏中持续56天或更长时间。组织细胞培养1周后，从血液，淋巴结或脾淋巴细胞中回收了病毒，但是只有在接种后48小时内摘除器官，才能直接在细胞中检测到病毒。为了确定病毒如何在低浓度下持续存在并作为潜伏感染，首先通过三种技术选择脾脏进行研究：脾脏的均质化，悬挂的梅特兰片段培养物和脾脏细胞的体外培养。最后的步骤显示感染豚鼠后56天，病毒在成纤维细胞样细胞（可能是巨噬细胞或网状内皮细胞）中传播。使用其他方法，仅在接种后的前两天内发现病毒。

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