Isolation and antigenic characteristics of axial filaments from the Reiter Treponeme.

P H Hardy; W R Fredericks; E E Nell

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Isolation and antigenic characteristics of axial filaments from the Reiter Treponeme.

机译：Reiter Treponeme的轴向细丝的分离和抗原特性。

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Axial filaments were isolated and purified from Reiter treponemes after detergent solubilization of the cells" outer envelope. The axial filaments were separated from the spirochetal cells by shearing, purified by density gradient centrifugation, and fragmented by ultrasonication. Acrylamide gel electrophoresis of dissociated filaments revealed two major protein bands. Gel diffusion precipitin tests and immunoelectrophoresis between a purified axial filament suspension and anti-Reiter treponeme serum gave a single precipitin line. Checkerboard complement fixation tests also gave results consistent with a single antigen-antibody system. Tests with immune sera to other cultivable spirochetes were positive with some and negative with others. In addition, strongly positive reactions were obtained in complement fixation and precipitin tests with sera from rabbits and humans with syphilis and other treponematoses. However, both serological tests gave reactions of partial identity between the antigen(s) of Reiter treponeme axial filaments and those of the pathogenic treponemes. It was concluded from these studies that the axial filaments were probably the cellular locus of the so-called "Reiter protein" antigen of syphilis serology.

机译：在去污剂溶解细胞外被膜后，从Reiter座膜分离并纯化轴向细丝。通过剪切将轴向细丝与螺旋形细胞分离，通过密度梯度离心纯化，并通过超声破碎。分离的细丝的丙烯酰胺凝胶电泳显示出两个纯化的轴向细丝悬浮液和抗Reiter treponeme血清之间的凝胶扩散沉淀蛋白测试和免疫电泳得到一条沉淀蛋白线，棋盘补体固定测试也得到与单一抗原抗体系统一致的结果，其他人的免疫血清测试可培养的螺旋体在某些情况下是阳性的，而在另一些情况下则是阴性的，此外，在患有梅毒和其他梅毒的家兔和人的血清中进行补体固定和沉淀蛋白测试时，获得了强烈的阳性反应。 Reiter截短杆菌轴向细丝的抗原和致病性截短的抗原。从这些研究得出的结论是，轴向细丝可能是梅毒血清学的所谓“ Reiter蛋白”抗原的细胞基因座。

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《Infection and immunity》 |1975年第2期|共7页
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P H Hardy; W R Fredericks; E E Nell;
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中图分类医学免疫学;
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1. Isolation and antigenic characteristics of axial filaments from the Reiter Treponeme. [J] . P H Hardy, W R Fredericks, E E Nell Infection and immunity . 1975,第2期

机译：Reiter Treponeme的轴向细丝的分离和抗原特性。
2. Counterimmunoelectrophoresis of Reiter Treponeme Axial filaments as a diagnostic test for syphilis. [J] . E E Nell, P H Hardy Journal of Clinical Microbiology . 1978,第2期

机译：重孕纤维轴丝的逆压力电泳作为梅毒诊断试验。
3. Isolation of Chlamydia trachomatis or Ureaplasma urealyticum from the synovial fluid of patients with Reiter's syndrome [J] . Pavlica Ljiljana, Dra?kovi? Nada, Kulji?-Kapulica Nada, Vojnosanitetski Pregled . 2003,第1期

机译：从瑞特氏综合征患者滑液中分离出沙眼衣原体或解脲脲原体
4. Strain characteristic of filament wound composite cylinder under axial loading [C] . Weidong Wen, Jian Li, Haitao Cui International Conference on Advances in Materials and Manufacturing Processes . 2012

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5. Multi-axial active isolation for seismic protection of buildings . [D] . Chang, Chia-Ming. 2011

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6. Isolation and antigenic characteristics of axial filaments from the Reiter Treponeme. [O] . P H Hardy Jr, W R Fredericks, E E Nell 1975

机译：Reiter Treponeme的轴向细丝的分离和抗原特性。
7. Enzyme linked immunosorbent assays with Treponema pallidum or axial filament of T phagedenis biotype Reiter as antigen: evaluation as screening tests for syphilis. [O] . van Eijk, R V, Menke, H E, Tideman, G J, 1986

机译：以苍白密螺旋体或pha phagedenis生物型Reiter的轴向丝作为抗原的酶联免疫吸附测定：评估为梅毒筛查试验。
8. Isolation and Characterization of Surface Antigens from Schistosoma Mansoni. II. Antigenicity of Radiolabeled Proteins from Adult Worms [R] . Hayunga, E. G., Murrell, K. D., Taylor, D. W., 1979

机译：血吸虫（schistosoma mansoni）表面抗原的分离与鉴定。 II。成体蠕虫放射性标记蛋白的抗原性

Isolation and antigenic characteristics of axial filaments from the Reiter Treponeme.

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