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首页> 外文期刊>Infection and immunity >Measurement of antibody to influenza virus neuraminidase by single radial hemolysis in agarose gels.
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Measurement of antibody to influenza virus neuraminidase by single radial hemolysis in agarose gels.

机译：在琼脂糖凝胶中通过单次径向溶血测定流感病毒神经氨酸酶抗体。

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摘要

A simple method of assaying anti-influenza neuraminidase antibodies in human sera was described. Suitable antigenic hybrid viruses were adsorbed to sheep erythrocytes, which were then incorporated into agarose gels. When sera were introduced into wells cut in the gels, zones of hemolysis were observed in the neighborhood of those containing neuraminidase antibodies. There was a direct relationship between zone size and antibody titer. No purification of adsorbed viruses was necessary. The test was rapid, required very simple reagents, gave results that agreed well with those given by conventional techniques, and appeared to be the most sensitive of four methods evaluated. Studies of cross-reactions by hyperimmune sera against homologous and heterologous neuraminidases and of absorption of neuraminidase antibodies from human sera indicated a high degree of specificity. The technique seems to be suitable for large-scale epidemiological investigations.
机译：描述了一种测定人血清中抗流感神经氨酸酶抗体的简单方法。将合适的抗原性杂种病毒吸附到绵羊红细胞上，然后将其掺入琼脂糖凝胶中。当将血清引入凝胶切开的孔中时，在含有神经氨酸酶抗体的区域附近观察到溶血区域。区域大小和抗体效价之间存在直接关系。无需纯化吸附的病毒。该测试是快速的，需要非常简单的试剂，其结果与常规技术所给出的结果非常吻合，并且似乎是所评估的四种方法中最敏感的。超免疫血清对同源和异源神经氨酸酶的交叉反应以及从人血清吸收神经氨酸酶抗体的研究表明高度特异性。该技术似乎适用于大规模流行病学调查。

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