...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Serotype-Nonspecific Protection Induced by Ribonucleic Acid Isolated from the Ribosomal Vaccine of Pseudomonas aeruginosa
【24h】

Serotype-Nonspecific Protection Induced by Ribonucleic Acid Isolated from the Ribosomal Vaccine of Pseudomonas aeruginosa

机译：铜绿假单胞菌核糖体疫苗核糖核酸诱导的血清型非特异性保护。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A ribosomal vaccine of Pseudomonas aeruginosa and a vaccine containing purified lipopolysaccharide (LPS) were compared with respect to their capacity to protect mice against a lethal challenge with P. aeruginosa. The route of vaccination appeared to be important for the protective activity of the ribosomal vaccine. Optimal protection was measured if both the immunizing and the challenge injection were given intraperitoneally. The ribosomal vaccine protected mice as early as 1 day after vaccination, and the protection lasted at least 6 days. LPS-specific antibodies were detectable 6 but not 2 days after vaccination. The ribosomal vaccine protected mice also against a heterologous serotype of Pseudomonas. Injection of purified LPS did not protect mice earlier than at day 3, and the protection induced by LPS was serotype specific. Ribonucleic acid (RNA) isolated from the ribosomal vaccine had the same protective properties as the ribosomes. RNA induced serotype-nonspecific protection as quickly as 1 day after injection, and the protection lasted at least 6 days. However, the capacity to induce antibodies to LPS was lost or reduced. It is concluded that the serotype-nonspecific protection induced by RNA and the serotype-specific protection induced by LPS are due to different mechanisms. Experiments with combined vaccines containing RNA and LPS demonstrated that the addition of RNA to LPS resulted in a slight increase in LPS-specific antibodies. The data presented indicate that both the serotype-specific protection induced by LPS and the serotype-nonspecific protection induced by RNA contribute to the protective activity of the ribosomal vaccine.
机译：比较了铜绿假单胞菌的核糖体疫苗和含有纯化的脂多糖（LPS）的疫苗在保护小鼠免受 P致死性攻击方面的能力。铜绿疫苗。疫苗接种途径对于核糖体疫苗的保护活性似乎很重要。如果腹膜内同时给予免疫注射和攻击注射，则可测量最佳保护。核糖体疫苗最早在接种疫苗后1天就对小鼠产生了保护，并且这种保护至少持续了6天。接种疫苗后6天即可检测到LPS特异性抗体，但检测不到2天。核糖体疫苗还可以保护小鼠免受假单胞菌的异源血清型的侵害。纯化LPS的注射并不能在第3天之前保护小鼠，并且LPS诱导的保护具有血清型特异性。从核糖体疫苗中分离出的核糖核酸（RNA）具有与核糖体相同的保护特性。 RNA注射后最快1天即可诱导血清型非特异性保护，这种保护至少持续6天。然而，诱导针对LPS的抗体的能力丧失或降低。结论是RNA诱导的血清型非特异性保护和LPS诱导的血清型非特异性保护是由于不同的机制引起的。用含有RNA和LPS的联合疫苗进行的实验表明，向LPS添加RNA会导致LPS特异性抗体略有增加。呈现的数据表明，LPS诱导的血清型特异性保护和RNA诱导的血清型非特异性保护都有助于核糖体疫苗的保护活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号