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首页> 外文期刊>Infection and immunity >Course of coccidioidomycosis in intratracheally infected guinea pigs.
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Course of coccidioidomycosis in intratracheally infected guinea pigs.

机译：气管内感染豚鼠的球虫病病程。

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Two hundred Hartley-inbred guinea pigs were infected intratracheally with 50 viable arthrospores of Coccidioides immitis. At weeks 1 through 10 postinfection, groups of 20 guinea pigs were assayed for skin test, macrophage migration inhibitory factor (MIF), and lymphocyte transformation (LT) responses to coccidioidin. Forty-eight hours after skin testing and just before MIF and LT assays, blood was obtained for complement-fixing (CF) antibody titers and the animals were autopsied to assess the extent of fungal dissemination. Immunological assays established that skin tests and MIF responses converted within 3 weeks of infection. LT responses were not demonstrable until week 5. Dissemination of C. immitis to the liver or spleen was an early event, with 21% of guinea pigs positive by week 2 and 70% positive by week 5. CF antibody titers were demonstrable at week 5, increased logarithmically through week 7, then increased at a slower rate thereafter. Concomitant with the decreased rate of antibody production, guinea pigs began to clear C. immitis from their extrapulmonary tissues. Skin test responses peaked at 6 weeks postinfection when CF antibody titers were less than or equal to 1:16 and then plateaued with increased CF titers. Although this overall immunological profile is consistent with the disease in humans, there was not a direct correlation between CF antibody titer and dissemination to the liver or spleen, nor was there an inverse correlation between CF antibody titers and skin test or MIF responses. Rather, CF antibody titers and cell-mediated immune responses were equally demonstrable in guinea pigs with disseminated or nondisseminated disease.
机译：200只哈特利近交系豚鼠在气管内感染了50种活的球虫球菌性炎球菌。在感染后的第1至10周，对20只豚鼠进行了皮肤测试，巨噬细胞迁移抑制因子（MIF）和对球孢菌素的淋巴细胞转化（LT）反应的测定。皮肤测试后四十八小时，就在MIF和LT检测之前，采集血液以检测补体固定（CF）抗体滴度，并对动物进行尸检以评估真菌传播的程度。免疫学测定表明，皮肤测试和MIF反应在感染后3周内发生了转化。直到第5周才能证实LT的反应是早期传播到肝或脾中的梭状芽胞杆菌，在第2周时有21％的豚鼠为阳性，在第5周时为70％的阳性。 ，直到第7周都呈对数增长，然后以较慢的速度增长。伴随抗体产生率的降低，豚鼠开始从其肺外组织清除念珠菌。当CF抗体滴度小于或等于1:16时，皮肤测试反应在感染后6周达到峰值，然后随着CF滴度增加而达到稳定。尽管这种总体免疫学特征与人类疾病相符，但CF抗体滴度与向肝脏或脾脏的扩散之间没有直接相关性，CF抗体滴度与皮肤测试或MIF反应之间也没有负相关性。相反，在已播散或未播散疾病的豚鼠中，CF抗体滴度和细胞介导的免疫反应同样可证明。

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