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首页> 外文期刊>Infection and immunity >Stimulation of human gamma interferon production by diterpene esters.
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Stimulation of human gamma interferon production by diterpene esters.

机译：二萜酯刺激人γ干扰素的产生。

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The diterpene ester 12-O-tetradecanoylphorbol-13-acetate (TPA) and several structurally related compounds were tested for their ability to stimulate interferon (IFN) production in primary cultures of human leukocytes. In cultures of Ficoll-Hypaque-purified mononuclear cells, TPA treatment alone induced only low levels of IFN, but TPA pretreatment of cells caused significant enhancement of IFN yields produced with phytohemagglutinin or several other T cell mitogens. In cultures of unprocessed cells derived from plateletpheresis residues or buffy coats, TPA treatment alone induced high levels of IFN and costimulation with TPA and phytohemagglutinin produced some further enhancement of IFN production. Phorbol 12,13-dibutyrate was comparable to TPA in its ability to enhance phytohemagglutinin-induced IFN production. Several other phorbol ester analogs were also active, but maximal stimulation occurred only at higher drug concentrations. Mezerein, a structurally related diterpene ester, was at least as active as TPA in stimulating IFN production in either Ficoll-Hypaque-purified or unprocessed cells. IFN produced after stimulation with TPA or mezerein, singly or in combination with phytohemagglutinin, had several properties characteristic of IFN-gamma, e.g., it was largely inactivated by dialysis at pH 2, or after exposure to sodium dodecyl sulfate, whereas it was not neutralized by antibody to IFN-alpha and IFN-beta. The stimulatory effect of diterpene esters has proved helpful in producing IFN-gamma for physicochemical analysis and other studies.
机译：测试了双萜酯12-O-十四烷酰基phorbol-13-乙酸酯（TPA）和几种与结构相关的化合物刺激人白细胞原代培养物中干扰素（IFN）产生的能力。在Ficoll-Hypaque纯化的单核细胞培养物中，单独的TPA处理仅诱导低水平的IFN，但是细胞的TPA预处理导致植物血凝素或其他几种T细胞促细胞分裂剂产生的IFN产量显着提高。在未经处理的细胞培养物中衍生自血小板减少症残基或血沉棕黄层，单用TPA处理即可诱导高水平的IFN，并与TPA和植物血凝素共刺激可进一步提高IFN的产生。 Phorbol 12,13-dibutyrate在增强植物血凝素诱导的IFN产生能力上与TPA相当。其他几种佛波酯类似物也具有活性，但是最大刺激仅在较高的药物浓度下发生。 Mezerein是一种结构相关的二萜酯，在刺激Ficoll-Hypaque纯化或未加工的细胞中，在刺激IFN产生方面至少与TPA活性相同。用TPA或美西瑞因刺激后，单独或与植物血凝素结合后产生的IFN具有IFN-γ的几种特性，例如，在pH 2下透析或暴露于十二烷基硫酸钠后大部分被灭活，而并未被中和。通过针对IFN-α和IFN-β的抗体。已证明二萜酯的刺激作用有助于产生用于物理化学分析和其他研究的IFN-γ。

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