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首页> 外文期刊>Infection and immunity >Gamma interferon production by combinations of human peripheral blood lymphocytes, monocytes, and cultured macrophages.
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Gamma interferon production by combinations of human peripheral blood lymphocytes, monocytes, and cultured macrophages.

机译：人类外周血淋巴细胞，单核细胞和培养的巨噬细胞的组合产生的γ干扰素。

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Mitogen-induced interferon (IFN) production was studied using human peripheral blood mononuclear cells and subpopulations of lymphocytes, monocytes, and cultured macrophages. Cell populations were prepared in suspension to permit quantitative analysis of the interactions among different cell types. After stimulation by staphylococcal enterotoxin A, nylon column-purified lymphocytes produced only 5% as much IFN as the peripheral blood mononuclear cells from which they were prepared. When lymphocytes were supplemented with as little as 2% monocytes, IFN production increased two- to eightfold; with the addition of up to 20% monocytes, IFN production increased further, to levels approximating those of peripheral blood mononuclear cells. Monocytes alone produced no or very little IFN. Macrophages were derived from monocytes by culturing in vitro for 7 days. The addition of 2 to 5% autologous macrophages augmented IFN production to the same extent as 2 to 5% monocytes. However, more macrophages consistently resulted in less, rather than more, IFN, so that lymphocytes with 20% monocytes produced three- to eightfold more IFN than did lymphocytes with 20% macrophages. Thus, whereas the addition of monocytes over a broad dose-response range (2 to 20%) progressively augmented IFN production, macrophages showed an optimal effect at 2 to 5%, with higher percentages being inhibitory. The IFN induced by stimulation with staphylococcal enterotoxin A was characterized as IFN-gamma by its resistance to neutralization by antibody to IFN- alpha and its inability to induce antiviral protection in embryonic bovine trachea cells.
机译：使用人类外周血单核细胞以及淋巴细胞，单核细胞和培养的巨噬细胞亚群研究了丝裂原诱导的干扰素（IFN）的产生。制备悬液中的细胞群，以定量分析不同细胞类型之间的相互作用。在葡萄球菌肠毒素A刺激后，尼龙柱纯化的淋巴细胞产生的IFN仅为制备它们的外周血单核细胞的5％。当淋巴细胞补充少至2％的单核细胞时，IFN的产量增加了2到8倍。通过添加多达20％的单核细胞，IFN的产生会进一步增加，达到接近外周血单核细胞的水平。单核细胞单独不产生或很少产生IFN。巨噬细胞通过体外培养7天而从单核细胞衍生而来。添加2至5％的自体巨噬细胞可将IFN的产生增加到与2至5％的单核细胞相同的程度。然而，更多的巨噬细胞持续产生更少而不是更多的IFN，因此具有20％单核细胞的淋巴细胞产生的IFN比具有20％巨噬细胞的淋巴细胞产生的干扰素高三到八倍。因此，尽管在较宽的剂量反应范围（2％至20％）内添加单核细胞会逐渐增加IFN的产生，但巨噬细胞在2％至5％时表现出最佳效果，而抑制作用的百分比更高。由葡萄球菌肠毒素A刺激诱导的IFN以其对IFN-α抗体的中和抗性以及无法在胚牛气管细胞中诱导抗病毒保护而被表征为IFN-γ。

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