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首页> 外文期刊>Infection and immunity >Mechanism of the bactericidal action of myeloperoxidase: increased permeability of the Escherichia coli cell envelope.
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Mechanism of the bactericidal action of myeloperoxidase: increased permeability of the Escherichia coli cell envelope.

机译：髓过氧化物酶杀菌作用的机制：大肠杆菌细胞膜的通透性增加。

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The killing of Escherichia coli by isolated human myeloperoxidase plus hydrogen peroxide plus chloride ions was shown to proceed via an increased permeability of the bacterial cell wall. A correlation between the extent of the increased permeability and the number of surviving colony-forming units was found (P less than 0.0005). The same phenomenon was observed with isolated human neutrophils. The permeability increase was shown to be limited, because low-molecular-weight substrate became freely permeant, but the bacteria retained their permeability barrier for protein. Furthermore, disruption of the permeability barrier was followed by destruction of cytoplasmic protein. The active antibacterial agent was probably hypochlorous acid, the direct product of the system, rather than singlet oxygen, the nonenzymic product of hypochlorous acid and hydrogen peroxide. This is concluded from the fact that the myeloperoxidase system could be mimicked by hypochlorous acid, whereas conditions that favor formation of singlet oxygen did not enhance this effect. The relevance of the system for killing of bacteria at neutral pH is discussed.
机译：分离出的人髓过氧化物酶加过氧化氢加氯离子对大肠杆菌的杀灭作用是通过增加细菌细胞壁的通透性来进行的。发现渗透性增加的程度与存活的菌落形成单位数目之间存在相关性（P小于0.0005）。对于分离的人嗜中性粒细胞，观察到相同的现象。由于低分子量底物可以自由渗透，因此细菌的通透性增加受到限制，但是细菌保留了其对蛋白质的通透性屏障。此外，破坏通透性屏障后破坏细胞质蛋白。活性抗菌剂可能是系统的直接产物次氯酸，而不是单线态氧，即次氯酸和过氧化氢的非酶产物。这可以从以下事实得出结论：髓过氧化物酶系统可以被次氯酸模拟，而有利于单线态氧形成的条件并不能增强这种作用。讨论了在中性pH值下杀死细菌的系统的相关性。

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