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首页> 外文期刊>Infection and immunity >Identification and antigenic characterization of virulence-associated, plasmid-coded proteins of Shigella spp. and enteroinvasive Escherichia coli.
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Identification and antigenic characterization of virulence-associated, plasmid-coded proteins of Shigella spp. and enteroinvasive Escherichia coli.

机译：志贺菌属的毒力相关质粒编码蛋白的鉴定和抗原表征。和肠溶性大肠杆菌

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Seven plasmid-coded polypeptides, designated a through g, were identified by two-dimensional nonequilibrium pH gradient electrophoresis of radiolabeled extracts from minicells of virulent Shigella flexneri serotypes 2a and 5 and enteroinvasive Escherichia coli O143. These polypeptides were deemed to be products of 140-megadalton (MDa) virulence-associated plasmids because they were not synthesized in minicells which were not harboring a 140-MDa plasmid or in minicells which were carrying an F lac plasmid of the same incompatibility group. Synthesis of these polypeptides was repressed in minicells incubated at 30 degrees C and in minicells isolated from a noninvasive opaque colonial variant, even though these strains harbored a 140-MDa plasmid. Enriched fractions of polypeptides b, c, and d were obtained from S. flexneri serotype 5 by preparative isoelectric focusing, and polyclonal rabbit antisera recognizing each polypeptide were raised. These antisera were able to detect cross-reacting plasmid-coded polypeptide antigens in S. flexneri serotype 3, Shigella sonnei, and enteroinvasive E. coli O143. In addition, Western blots of minicell extracts from S. flexneri serotype 5 or E. coli O143 indicated that plasmid-coded polypeptides a through d were recognized by convalescent antiserum from a monkey infected with S. flexneri serotype 2a.
机译：通过对强毒志贺氏菌血清型2a和5以及肠道侵袭性大肠杆菌O143的小细胞进行放射性标记提取物的二维非平衡pH梯度电泳，鉴定了7种质粒编码的多肽，命名为a至g。这些多肽被认为是140百万道尔顿（MDa）毒力相关质粒的产物，因为它们不是在不包含140-MDa质粒的小细胞中或在携带相同不相容基团的F lac质粒的小细胞中合成的。这些多肽的合成在30°C下温育的小细胞和从非侵入性不透明菌落变体分离的小细胞中受到抑制，即使这些菌株具有140-MDa质粒也是如此。通过制备等电聚焦从弗氏链球菌血清型5获得富集的多肽b，c和d的级分，并产生识别每种多肽的多克隆兔抗血清。这些抗血清能够检测弗氏链球菌血清型3，重症志贺氏菌和肠侵染性大肠杆菌O143中交叉反应的质粒编码的多肽抗原。另外，来自弗氏链球菌血清型5或大肠杆菌O143的小细胞提取物的蛋白质印迹表明，来自被弗氏链球菌血清型2a感染的猴子的恢复期抗血清可识别质粒编码的多肽a至d。

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