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首页> 外文期刊>Journal of Clinical Microbiology >Characterization of virulence marker antigen of Shigella spp. and enteroinvasive Escherichia coli.
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Characterization of virulence marker antigen of Shigella spp. and enteroinvasive Escherichia coli.

机译:志贺氏菌的毒力标记抗原的表征。和肠溶性大肠杆菌

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Antisera produced in rabbits immunized with an enteroinvasive O143 strain of Escherichia coli were absorbed with an avirulent derivative of the same strain. The resulting sera have been previously shown to recognize enteroinvasive pathogens when used in an enzyme-linked immunosorbent assay. In the current study, Western blots (immunoblots) showed that such an absorbed rabbit antiserum recognized two proteins (IpaB and IpaC) which are encoded by a large, virulence-associated plasmid. These proteins are the apparent constituents of the virulence marker antigen which is expressed by shigellae and enteroinvasive E. coli.
机译:用大肠杆菌的肠溶性O143菌株免疫的兔子产生的抗血清被相同菌株的无毒衍生物吸收。先前已证明,所得的血清在用于酶联免疫吸附测定中时可识别肠侵袭性病原体。在当前的研究中,Western印迹(免疫印迹)表明,这种吸收的兔抗血清可以识别两种蛋白质(IpaB和​​IpaC),这两种蛋白质是由与毒力相关的大质粒编码的。这些蛋白质是由志贺氏菌和肠侵袭性大肠杆菌表达的毒性标记抗原的表观组成。

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