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首页> 外文期刊>Infection and immunity >Immunoelectron microscopy of lipopolysaccharide in Chlamydia trachomatis.
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Immunoelectron microscopy of lipopolysaccharide in Chlamydia trachomatis.

机译:沙眼衣原体中脂多糖的免疫电子显微镜检查。

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Monoclonal antibodies (MAb) specific for Chlamydia trachomatis lipopolysaccharide (LPS) and major outer membrane protein (MOMP) were used for immunoelectron microscopy analysis. MAb specific for MOMP showed strong reaction with the chlamydial surface, whereas MAb specific for LPS showed strong association of gold particles with the periphery of the chlamydial body. After fixation of the chlamydia cells, the reactivity was, however, similar to the anti-MOMP reactivity. Enzyme-linked immunosorbent assay showed that MAb specific for LPS could remove LPS from the chlamydial membrane.
机译:特异于沙眼衣原体脂多糖(LPS)和主要外膜蛋白(MOMP)的单克隆抗体(MAb)用于免疫电子显微镜分析。 MOMP特异的MAb显示与衣原体表面强烈反应,而LPS特异的MAb显示金颗粒与衣原体外围紧密结合。固定衣原体细胞后,反应性类似于抗MOMP反应性。酶联免疫吸附试验表明,针对LPS的单克隆抗体可以从衣原体膜中去除LPS。

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