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首页> 外文期刊>Infection and immunity >Phorbol myristate acetate modulates calcium ion-dependent superoxide anion generation induced by a monoclonal antibody raised against polymorphonuclear leukocytes.
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Phorbol myristate acetate modulates calcium ion-dependent superoxide anion generation induced by a monoclonal antibody raised against polymorphonuclear leukocytes.

机译：醋酸肉豆蔻酸乙酸酯调节由针对多形核白细胞的单克隆抗体诱导的钙离子依赖性超氧阴离子的产生。

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We used a monoclonal antibody, YI 51, raised against human polymorphonuclear leukocytes (PMN) to induce superoxide anion (O2-) generation in cells. Although YI 51 alone played only a small part in inducing O2- generation in PMN, the amount of O2- generation induced in 5 X 10(5) PMN was 3.7 to 5.5 nmol/min when F(ab')2 fragments of rabbit anti-mouse immunoglobulin antibody were added as a cross-linking agent. This O2- -inducing activity was high compared with that of wheat germ agglutinin (WGA), insoluble immunoglobulin G immune complexes (IC), or phorbol myristate acetate (PMA). The binding of YI 51 and soluble immunoglobulin G IC to PMN was not reciprocally inhibitory, indicating that YI 51 does not interfere with ligand binding to the Fc receptor-binding site. In the absence of calcium ion (Ca2+), O2- generation induced by YI 51 decreased to 10 to 20% of that in the presence of Ca2+. In contrast, O2- generation in response to WGA, IC, or PMA under Ca2+-free conditions was not affected. When PMN were pretreated with low concentrations of PMA (10(-10) to 10(-9) M), the amount of O2- generation by the cells in response to YI 51 in Ca2+-free buffer was enhanced in a concentration-dependent manner. It also equaled the O2- generated by the cells in buffer containing Ca2+. In cells pretreated with PMA, the amount of O2- induced by WGA was enhanced two- to threefold over that in untreated cells. In contrast, there was no augmentation over untreated cells with stimulation by IC. These results suggest that YI 51, IC, and WGA induce O2- generation in human PMN in different manners.
机译：我们使用抗人多形核白细胞（PMN）的单克隆抗体YI 51诱导细胞中的超氧阴离子（O2-）生成。尽管单独的YI 51在诱导PMN中O2-生成中仅发挥很小的作用，但是当兔抗F（ab'）2片段在5 X 10（5）PMN中诱导的O2-生成量为3.7至5.5 nmol / min。添加小鼠免疫球蛋白抗体作为交联剂。与小麦胚芽凝集素（WGA），不溶性免疫球蛋白G免疫复合物（IC）或佛波肉豆蔻酸酯乙酸酯（PMA）相比，这种O2诱导活性较高。 YI 51和可溶性免疫球蛋白G IC与PMN的结合没有相互抑制，这表明YI 51不会干扰配体与Fc受体结合位点的结合。在没有钙离子（Ca2 +）的情况下，由YI 51诱导的O2生成量减少到有Ca2 +存在时的10％至20％。相反，在无Ca2 +的条件下，响应WGA，IC或PMA的O2生成不会受到影响。当用低浓度的PMA（10（-10）至10（-9）M）预处理PMN时，在无浓度Ca2 +的缓冲液中，细胞响应YI 51产生的O2量会增加方式。它也等于细胞在含Ca2 +的缓冲液中产生的O2-。在用PMA预处理的细胞中，由WGA诱导的O2量比未处理的细胞增加了2到3倍。相反，通过IC刺激，未处理的细胞没有增加。这些结果表明，YI 51，IC和WGA以不同的方式诱导人PMN中O2的产生。

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